Polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and polynucleotides encoding same

Patent metadata

Field	Value
Publication number	US-9562222-B2
Application number	US-201214357902-A
Country	US
Kind code	B2
Filing date	Nov 16, 2012
Priority date	Nov 18, 2011
Publication date	Feb 7, 2017
Grant date	Feb 7, 2017

Abstract

The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

First claim

The invention claimed is:

1. A recombinant host cell comprising a polynucleotide encoding a polypeptide having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity, wherein
(a) the polypeptide has at least 90% sequence identity to amino acids 23 to 950 of SEQ ID NO: 2, amino acids 23 to 868 of SEQ ID NO: 4, amino acids 20 to 860 of SEQ ID NO: 6, or amino acids 20 to 737 of SEQ ID NO: 8 or the polypeptide is a fragment of amino acids 23 to 950 of SEQ ID NO: 2, amino acids 23 to 868 of SEQ ID NO: 4, amino acids 20 to 860 of SEQ ID NO: 6, or amino acids 20 to 737 of SEQ ID NO: 8, and
(b) the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide.

2. A method of producing a polypeptide having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity, comprising:
(a) cultivating the host cell of claim 1 under conditions conducive for production of the polypeptide; and
(b) recovering the polypeptide.

3. The method of claim 2 , wherein the polypeptide has at least 90% sequence identity to amino acids 23 to 950 of SEQ ID NO: 2.

4. The method of claim 2 , wherein the polypeptide has at least 95% sequence identity to amino acids 23 to 950 of SEQ ID NO: 2.

5. The method of claim 2 , wherein the polypeptide has at least 97% sequence identity to amino acids 23 to 950 of SEQ ID NO: 2.

6. The method of claim 2 , wherein the polypeptide comprises amino acids 23 to 950 of SEQ ID NO: 2.

7. The method of claim 2 , wherein the polypeptide has at least 90% sequence identity to amino acids 23 to 868 of SEQ ID NO: 4.

8. The method of claim 2 , wherein the polypeptide has at least 95% sequence identity to amino acids 23 to 868 of SEQ ID NO: 4.

9. The method of claim 2 , wherein the polypeptide has at least 97% sequence identity to amino acids 23 to 868 of SEQ ID NO: 4.

10. The method of claim 2 , wherein the polypeptide comprises amino acids 23 to 868 of SEQ ID NO: 4.

11. The method of claim 2 , wherein the polypeptide has at least 90% sequence identity to amino acids 20 to 860 of SEQ ID NO: 6.

12. The method of claim 2 , wherein the polypeptide has at least 95% sequence identity to amino acids 20 to 860 of SEQ ID NO: 6.

13. The method of claim 2 , wherein the polypeptide has at least 97% sequence identity to amino acids 20 to 860 of SEQ ID NO: 6.

14. The method of claim 2 , wherein the polypeptide comprises amino acids 20 to 860 of SEQ ID NO: 6.

15. The method of claim 2 , wherein the polypeptide has at least 90% sequence identity to amino acids 20 to 737 of SEQ ID NO: 8.

16. The method of claim 2 , wherein the polypeptide has at least 95% sequence identity to amino acids 20 to 737 of SEQ ID NO: 8.

17. The method of claim 2 , wherein the polypeptide has at least 97% sequence identity to amino acids 20 to 737 of SEQ ID NO: 8.

18. The method of claim 2 , wherein the polypeptide comprises amino acids 20 to 737 of SEQ ID NO: 8.

19. A process for degrading or converting a cellulosic material or xylan-containing material, comprising: treating the cellulosic material or xylan-containing material with an enzyme composition in the presence of the polypeptide having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity, wherein the polypeptide has at least 90% sequence identity to amino acids 23 to 950 of SEQ ID NO: 2, amino acids 23 to 868 of SEQ ID NO: 4, amino acids 20 to 860 of SEQ ID NO: 6, or amino acids 20 to 737 of SEQ ID NO: 8 or the polypeptide is a fragment of amino acids 23 to 950 of SEQ ID NO: 2, amino acids 23 to 868 of SEQ ID NO: 4, amino acids 20 to 860 of SEQ ID NO: 6, or amino acids 20 to 737 of SEQ ID NO: 8.

20. The process of claim 19 , further comprising recovering the degraded cellulosic material or xylan-containing material.

21. A process for producing a fermentation product, comprising:
(a) saccharifying a cellulosic material or xylan-containing material with an enzyme composition in the presence of the polypeptide having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity, wherein the polypeptide has at least 90% sequence identity to amino acids 23 to 950 of SEQ ID NO: 2, amino acids 23 to 868 of SEQ ID NO: 4, amino acids 20 to 860 of SEQ ID NO: 6, or amino acids 20 to 737 of SEQ ID NO: 8 or the polypeptide is a fragment of amino acids 23 to 950 of SEQ ID NO: 2, amino acids 23 to 868 of SEQ ID NO: 4, amino acids 20 to 860 of SEQ ID NO: 6, or amino acids 20 to 737 of SEQ ID NO: 8;
(b) fermenting the saccharified cellulosic material or xylan-containing material with one or more fermenting microorganisms to produce the fermentation product; and
(c) recovering the fermentation product from the fermentation.

Assignees

• Novozymes Inc
• Novozymes As

Inventors

• Morant Marc

Classifications

• C12P7/10

  substrate containing cellulosic material · CPC title

• C12N9/2482

  Endo-1,4-beta-xylanase (3.2.1.8) · CPC title

• C12P2203/00

  Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source · CPC title

• C12Y302/01008

  Endo-1,4-beta-xylanase (3.2.1.8) · CPC title

• C12P19/14

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title