Devices and methods for separating particles

Patent metadata

Field	Value
Publication number	US-9557250-B2
Application number	US-201313787874-A
Country	US
Kind code	B2
Filing date	Mar 7, 2013
Priority date	May 17, 2012
Publication date	Jan 31, 2017
Grant date	Jan 31, 2017

Abstract

Embodiments of the present disclosure provide for particle-imprinted polymer films, methods of making particle-imprinted polymer films, methods for separating particles, devices or systems for separating particles, and the like.

First claim

At least the following is claimed:

1. A particle-imprinted polymer film comprising: a single layer polymer film having indentations that have non-covalent chemical binding characteristics that preferentially capture at least one type of target particle via a direct non-covalent interaction with the at least one type of target particle, wherein the polymer film comprises a copolymer that includes polydimethylsiloxane (PDMS), wherein the polymer film is made from a prepolymer that includes a copolymer that has a specific affinity to characteristic components on the surface of an inactivated target particle, and wherein the copolymer is poly(diallydimethylammonium chloride) (PDADMAC) and PDMS.

2. A microfluidic device for separating target particles, comprising:
a sample holding area;
a second area including one or more particle-imprinted polymer films having a single layer polymer film having indentations that have non-covalent chemical binding characteristics that preferentially capture at least one type of target particle, wherein second area is in fluidic communication with the sample holding area via one or more inlets; and
a structure that is used to generate a centrifugal force, wherein the sample holding area and the second area are disposed on or are part of the structure, wherein each single layer polymer film is disposed on a substrate that is in a plane that is at an angle to the plane of the centrifugal force, wherein the centrifugal force is capable of causing a sample to move from the sample holding area into the second area.

3. The microfluidic device of claim 2 , wherein the centrifugal force is capable of causing the target particles to contact the single layer polymer film at a greater rate than if the sample were pressure driven to flow across the single layer polymer film.

4. The microfluidic device of claim 2 , wherein the sample holding area is positioned in the center of the structure and the second area is positioned adjacent the sample holding area in an area offset from the center of the structure.

5. The microfluidic device of claim 2 , wherein the target particles are selected from the group consisting of: active target particles, inactivated target particles, and a combination thereof.

Assignees

• Univ Leland Stanford Junior

Inventors

• Schirhagl Romana
• Zare Richard N
• Ren Kangning
• Banaei Niaz

Classifications

• G01N1/34 Primary

  Purifying; Cleaning {(processes or apparatus for extracting or separating nucleic acids from biological samples C12N15/1003)} · CPC title

• B01J20/268

  Polymers created by use of a template, e.g. molecularly imprinted polymers · CPC title

• G01N1/405 Primary

  by adsorption or absorption · CPC title

• B01L2400/0409

  centrifugal forces · CPC title

• B29C59/005

  characterised by the choice of material · CPC title