Enzyme containing liquid and delivery system for detection of analytes on surfaces

Patent metadata

Field	Value
Publication number	US-9556471-B2
Application number	US-50129606-A
Country	US
Kind code	B2
Filing date	Aug 9, 2006
Priority date	Aug 9, 2006
Publication date	Jan 31, 2017
Grant date	Jan 31, 2017

Abstract

An aqueous enzyme based sensor comprising one or more enzymes and at least one indicator compound capable of indicating a reaction of at least one of the enzymes with at least one target analyte, wherein the enzyme and the indicator are capable of being delivered, preferably by being sprayed, to a surface having the target analyte without sampling the surface. An aqueous enzyme based sensor is provided that is capable of detecting an enzyme specific substrate as the analyte. This aqueous enzyme based sensor further comprises of one or more enzyme specific substrates in which the sensor detects enzyme inhibition. The aqueous enzyme based sensor optionally comprises one or more of a light scattering additive(s), an adhesive polymer(s), a protein stabilizer(s), a protein stabilizing sugar(s), a surfactant(s), and a solvent(s), and combinations thereof. A dispensing system and a method of detecting a target chemical on a surface without sampling the surface is provided.

First claim

What is claimed is:

1. An aqueous enzyme based sensor comprising:
a mixture of moving liquid suspended in air comprising a composition of
two liquid mobilized enzymes that are acetyl cholinesterase and urease;
one or more cellulose adhesive polymers;
a solvent that is water;
two enzyme substrates that are acetylcholine and urea;
one or more light scattering additives; wherein said compostion has a constant acidic pH below 7.0 when the enzymes are uninhibited;
and optionally at least one additive comprising one or more of a protein stabilizer, a protein stabilizing sugar, and a surfactant, and combinations thereof; and
at least one indicator compound indicating an observable or machine readable reaction that occurs on a surface of an environment, and wherein said liquid enzyme(s), said adhesive polymer(s),said solvent, said light scattering additive(s) and said indicator compound(s) form a[n] sprayable aqueous enzyme based sensor mixture contained in a liquid dispensing device delivered to said surface by a spray nozzle in communication with said liquid dispensing device wherein said spray nozzle suspends the composition in air such that said aqueous enzyme based sensor mixture detects the presence or absence of at least one target analyte on said surface via an in situ reaction on said surface of an environment without the need for sampling of said surface and analyzing the sample elsewhere.

2. The aqueous enzyme based sensor of claim 1 wherein said indicator is a ph indicator dye that is selected from the group consisting of cresol red, phenol red, metacresol purple, neutral red, and curcumin.

3. The aqueous enzyme based sensor of claim 1 wherein said indicator is a pH sensitive fluorescent dye selected from the group consisting of carboxy-seminaphthorhodaflur and fluorescein.

4. The aqueous enzyme based sensor of claim 1 wherein said indicator is a chemiluminescent chemical that emits light upon enzymatic reaction selected from the group consisting of luminol and oxatane.

5. The aqueous enzyme based sensor of claim 1 wherein said indicator is a redox dye selected from the group consisting of guaiacol, 4-aminoantipyrine, and 3-(N-Ethyl-3-methylanilino)-2-hydroxypropanesulfonic acid.

6. The aqueous enzyme based sensor of claim 1 wherein said light scattering additive is an insoluble particulate metal oxide.

7. The aqueous enzyme based sensor of claim 6 wherein said metal oxide is a titanium dioxide or a silica-coated titanium dioxide.

8. The aqueous enzyme based sensor of claim 1 wherein said protein stabilizer is selected from the group consisting of poly(ethylene glycol), poly(N-vinyl pyrrolidone), dextran, poly(vinyl alcohol), and poly(ethylene imine).

9. The aqueous enzyme based sensor of claim 1 wherein said cellulose adhesive polymer is selected from the group consisting of carboxymethyl cellulose, and hydroxypropylmethyl cellulose.

10. The aqueous enzyme based sensor of claim 1 wherein said protein stabilizing sugar is selected from the group consisting of sucrose, trehalose, mannitol, xylitol, maltose, dextrose, and glucose.

11. The aqueous enzyme based sensor of claim 1 wherein said surfactant is selected from a group consisting of polyethylene glycol p-(1,1,3,3-tetramethylbutyl)-phenyl ether, polyoxyethylene (20) sorbitan monooleate, polyoxyethylene (20) sorbitan monolaureate, polyoxyethylene lauryl ether, lauramine oxide, and sodium di-2-ethyl-hexyl-sulphosuccinate.

12. The aqueous enzyme based sensor of claim 1 wherein said indicator senses at least one of a visible color change, a change in pH, oxidation state or substrate concentration.

13. The aqueous enzyme based sensor of claim 1 wherein said enzyme is an aqueous liquid.

14. The aqueous enzyme based sensor of claim 1 wherein said enzyme is a solid that is capable of being dissolved in water.

15. The aqueous enzyme based sensor of claim 1 wherein said indicator compound is an aqueous liquid.

16. The aqueous enzyme based sensor of claim 1 wherein said indicator compound is a solid that is capable of being dissolved in water.

17. The aqueous enzyme based sensor of claim 14 wherein said solid enzyme is a lyophilized powder.

18. The aqueous enzyme based sensor of claim 16 wherein said solid indicator compound is a lyophilized powder.

19. The aqueous enzyme based sensor of claim 17 wherein said enzyme lyophilized powder is prepared by lyophilizing a single enzyme or multiple enzymes in the presence of at least one stabilizing additive selected from the group consisting of a stabilizing polymer, a sugar, and combinations thereof.

20. The aqueous enzyme based sensor of claim 17 wherein said enzyme lyophilized powder is prepared by lyophilizing multiple enzymes individually and by mixing the individually lyophilized enzyme powders together to obtain the enzyme powder.

Assignees

• Lejuene Keith E
• Bacior Jennifer A
• Berberich Jason A
• Erbeldinger Markus
• Lele Bhalchandra Shripad
• Flir Detection Inc

Inventors

• Lejuene Keith E
• Bacior Jennifer A
• Berberich Jason A
• Erbeldinger Markus
• Lele Bhalchandra Shripad

Classifications

• C12Q1/28 Primary

  involving peroxidase · CPC title

• C12Q1/34

  involving hydrolase · CPC title

• C12Q1/37

  involving peptidase or proteinase · CPC title

• C12Q1/46

  involving cholinesterase · CPC title