Methods for making cytokine compositions from tissues using non-centrifugal methods

Patent metadata

Field	Value
Publication number	US-9556243-B2
Application number	US-201314050950-A
Country	US
Kind code	B2
Filing date	Oct 10, 2013
Priority date	Mar 15, 2013
Publication date	Jan 31, 2017
Grant date	Jan 31, 2017

Abstract

Apparatus and methods for generating a solution rich in interleukin-1 receptor antagonist from a tissue comprising cytokine-producing cells. The apparatus can include a filter used with a separation system. The separation system can include a float.

First claim

What is claimed is:

1. A system for the production of a solution rich in interleukin-1 receptor antagonist (IL-1ra) from a multiple component material, comprising:
a filter configured to filter a first component from the multiple component material and to allow a remaining multiple component material to pass;
a separation system to receive the remaining multiple component material, including;
a withdrawal port;
a separation container;
a buoy system positioned within the separation container;

wherein a second component comprising a buffy coat of the remaining multiple component material is operable to be separated from a third component of the remaining multiple component material within the separation container by the buoy system;
a withdrawal container connected to the filter;
non-centrifugal means for isolating cytokine-producing cells; and
a solid extraction material for producing a liquid volume comprising a solution rich in IL-1ra;
wherein the withdrawal container is configured to be connected to the withdrawal port and draw the second component from the separation container through the withdrawal port and through the filter into the withdrawal container;
wherein the first component includes cytokine-producing cells; and
wherein the second component is operable to separate the cytokine-producing cells from the filter to obtain a liquid volume comprising cytokine-producing cells.

2. The system of claim 1 , wherein the multiple component material is whole blood and the first component is white blood cells.

3. The system of claim 2 , wherein the second component is a buffy coat.

4. The system of claim 2 , wherein the third component is plasma.

5. The system of claim 1 , wherein the buoy system includes:
a first buoy member and a second buoy member, wherein the second component is contained between the first buoy member and the second buoy member after separation of the second component and the third component.

6. The system of claim 5 , wherein the first buoy member and the second buoy member are moveable relative to one another.

7. A system for the production of a solution rich in interleukin-1 receptor antagonist (IL-1ra) from a multiple component material, comprising:
a filter configured to filter a white blood cell component from the multiple component material and to allow a remaining multiple component material to pass;
a separation system to receive the remaining multiple component material, including;
a withdrawal port;
a separation container defining an internal volume;
a buoy system positioned in the internal volume of the separation container, wherein the buoy system includes a first buoy member and a second buoy member;

a withdrawal container configured to connect to the separation system to withdraw a second component comprising a buffy coat from the separation system, wherein the withdrawal container is connected to the filter;
non-centrifugal means for isolating cytokine-producing cells; and
a solid extraction material for producing a liquid volume comprising a solution rich in IL-1ra;
wherein the second component of the remaining multiple component material is operable to be separated from a third component of the remaining multiple component material within the separation container;
wherein the second component is able to be positioned between the first buoy member and the second buoy member;
wherein the third component is able to be positioned between the first buoy member and an end of the separation container
wherein the withdrawal container is configured to be connected to the withdrawal port and draw the second component from the separation container through the withdrawal port and through the filter into the withdrawal container;
wherein the first component comprises cytokine-producing cells; and
wherein the second component is operable to separate the cytokine-producing cells from the filter to obtain a liquid volume comprising cytokine-producing cells.

8. The system of claim 7 , wherein the filter is configured to be backwashed with the withdrawn second component.

9. The system of claim 7 , wherein the first buoy member is moveable relative to the second buoy member.

10. The system of claim 7 , wherein the filter is a size exclusion hollow fiber array.

Assignees

• Biomet Biologics Llc
• Biomet Biologies Llc

Inventors

• Leach Michael D
• Woodell-May Jennifer E
• Higgins Joel C
• O'Shaughnessey Krista

Classifications

• C07K1/34 Primary

  by filtration, ultrafiltration or reverse osmosis · CPC title

• C07K14/545

  IL-1 · CPC title

• A61K38/1808

  Epidermal growth factor [EGF] urogastrone · CPC title

• A61K2300/00

  Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00 · CPC title

• A61K38/1841

  Transforming growth factor [TGF] · CPC title