System and method for ionization of molecules for mass spectrometry and ion mobility spectrometry

Patent metadata

Field	Value
Publication number	US-9552973-B2
Application number	US-201113819487-A
Country	US
Kind code	B2
Filing date	Sep 1, 2011
Priority date	Sep 2, 2010
Publication date	Jan 24, 2017
Grant date	Jan 24, 2017

Abstract

An ionizing system includes a channel having an inlet disposed in a first pressure region and an outlet disposed in a second pressure region, a pressure of the first pressure region being greater than a pressure of the second pressure region. A heater is coupled to the channel and configured to heat the channel. A device is configured to introduce an analyte into the channel where the analyte is ionized.

First claim

What is claimed is:

1. An ionizing system, comprising:
a channel having an inlet disposed in a first pressure region and an outlet disposed in a second pressure region, a pressure of the first pressure region being greater than a pressure of the second pressure region;
a heater coupled to the channel and configured to heat the channel; and
a device for introducing at least one analyte molecule into the channel through which the at least one analyte molecule is ionized by the heat and pressure-differential applied in the channel to generate at least one analyte molecule ion.

2. The ionizing system of claim 1 , wherein the channel is defined by a transfer tube that is coupled to the heater such that the transfer tube is heated by the heater.

3. The ionizing system of claim 1 , wherein the channel is defined by the heater.

4. The ionizing system of claim 1 , wherein a skimmer is disposed over the inlet of the channel and defines an opening that is in fluid communication with the channel.

5. The ionizing system of claim 1 , wherein the device is a substrate on which the at least one analyte molecule is disposed.

6. The ionizing system of claim 5 , wherein the substrate is disposed adjacent to the inlet of the channel and is configured to be contacted by one of a physical object or electromagnetic energy.

7. The ionizing system of claim 1 , wherein the at least one analyte molecule is mixed with a matrix.

8. The ionizing system of claim 1 , wherein the device includes:
a container in which a solution including the at least one analyte molecule is disposed;
a conduit having a first end disposed in the container such that the first end is in contact with the solution including the at least one analyte molecule and a second end disposed adjacent to or within the inlet of the channel.

9. The ionizing system of claim 8 , further comprising an electrode disposed within the container and configured to apply a voltage to the solution including the at least one analyte molecule.

10. The ionizing system of claim 1 , wherein the device includes:
a container in which a solution including the at least one analyte molecule is disposed;
a mixing tube having a first end and a second end, the first end of the mixing tube receiving a capillary having a first end disposed within the solution, the second end of the mixing tube having a transfer tube extending therefrom such that an end of the transfer tube is disposed adjacent to or within the channel; and
a nebulizing tube configured to inject a nebulizing gas into the mixing tube.

11. The ionizing system of claim 10 , wherein a flow rate of the solution including the at least one analyte molecule is on the order of nanoliters per minute.

12. The ionizing system of claim 1 , wherein the channel is in fluid communication with one of a mass spectrometer or an ion mobility analyzer.

13. The ionizing system of claim 1 , wherein a gas at the inlet of the channel includes at least one of helium, argon, and nitrogen.

14. An ionizing method, comprising:
creating a pressure differential across a channel having a first end disposed in a first pressure and a second disposed in a second pressure, the first pressure being greater than the second pressure;
heating the channel;
receiving at least one analyte molecule in the channel; and
ionizing the at least one analyte molecule as the at least one analyte molecule moves through the channel, wherein the at least one analyte molecule is ionized by heat and the pressure-differential applied in the channel to generate at least one analyte molecule ion.

15. The ionizing method of claim 14 , further comprising applying a voltage to the at least one analyte molecule when the at least one analyte molecule is disposed in a solution within a container.

16. The ionizing method of claim 15 , wherein the analyte molecule is received within the channel from a conduit having a first end disposed in the container such that the first end is in contact with a solution including the analyte molecule and a second end is disposed adjacent to or within an inlet of the channel.

17. The ionizing method of claim 14 , wherein the at least one analyte molecule is received within the channel from a conduit having a first end disposed in the container such that the first end is in contact with a solution including the at least one analyte molecule and a second end is disposed within a mixing tube having a first end disposed within or adjacent to an inlet of the channel.

18. The ionizing method of claim 17 , further comprising:
receiving a nebulizing gas in the mixing tube; and
receiving the nebulizing gas and the at least one analyte molecule in the channel from an end of a transfer tube that extends from the first end of the mixing tube.

19. The ionizing method of claim 14 , further comprising receiving the at least one analyte molecule in an ionized form at a mass analyzer.

20. The ionizing method of claim 14 , wherein the at least one analyte molecule is received in the channel in a gas phase.

21. The ionizing method of claim 14 , wherein the at least one analyte molecule is mixed with one of a matrix or a solvent.

22. The ionizing method of claim 14 , wherein a gas at the inlet of the channel includes at least one of helium, argon, and nitrogen.

23. The ionizing system of claim 1 , further comprising an obstruction disposed along an axis defined by the channel.

24. The ionizing system of claim 23 , wherein the obstruction is disposed adjacent to the outlet of the channel.

Assignees

• Mcewen Charles Nehemiah
• Trimpin Sarah
• Pagnotti Vincent Salvatore
• Univ Of The Sciences In Philadelphia
• Univ Wayne State

Inventors

• Mcewen Charles Nehemiah
• Trimpin Sarah
• Pagnotti Vincent Salvatore

Classifications

• H01J49/10 Primary

  Ion sources; Ion guns · CPC title

• H01J49/0404

  Capillaries used for transferring samples or ions (electrospray nozzles H01J49/167) · CPC title

• H01J49/0468

  with means for heating or cooling the sample · CPC title