Method for detecting analyte in a bodily fluid, and dressing for implementing such a method

Patent metadata

Field	Value
Publication number	US-9551701-B2
Application number	US-201013520078-A
Country	US
Kind code	B2
Filing date	Dec 29, 2010
Priority date	Jan 4, 2010
Publication date	Jan 24, 2017
Grant date	Jan 24, 2017

Abstract

A method for detecting an analyte in a bodily fluid includes placing the detector in contact with at least one metabolite resulting from the metabolism or degradation of the analyte. The contact between the detector and the metabolite produces an optically detectable signal. The detection method includes the detection of the metabolite only in gas form, said metabolite in gas form coming from the evaporation of at least a part of said metabolite. The detection is also carried out without contact between the bodily fluid and the detector. A dressing for implementing the detection method is also described.

First claim

The invention claimed is:

1. A method for detecting an analyte in a bodily liquid fluid, comprising:
placing a dressing in contact with a human or animal skin, said skin excreting said bodily liquid fluid, said bodily liquid fluid including said analyte, said dressing including at least a detector, said detector being located above the human or animal skin;
waiting for said analyte to either degrade or metabolize, and in doing so release a Volatile Organic Compound (VOC), said VOC released from the analyte being in a liquid form;
waiting for said liquid form of the VOC to evaporate into a gaseous organic compound; and
detecting said gaseous organic compound with said detector, wherein
said detector is an optical detector, where the optical detector is configured such that the presence of a gaseous form of the VOC modifies optical properties of the optical detector, and
said detector does not contact said bodily liquid fluid.

2. The method of claim 1 , wherein said analyte is a bacteria.

3. The method of claim 1 , wherein said optical detector is a chromogene detector, which color changes when contacting said gaseous form of the VOC.

4. The method of claim 1 , wherein said optical detector is a fluorogene detector, which produces a fluorescent emission when contacting said gaseous form of the VOC.

5. The method of claim 1 , wherein said detector includes a probe, which is specific
to a predetermined Volatile Organic Compound released by the analyte, or
to a predetermined class of Volatile Organic Compounds released by the analyte.

6. The method of claim 1 , wherein said detector is isolated from said bodily fluid by a protection element, which prevents any contact between said bodily fluid and said detector.

7. The method of claim 1 , wherein
said detector includes
a support, which is permeable to the gaseous form of the VOC, and
a probe, which is incorporated into the support, said probe being sensitive to the gaseous form of the VOC.

8. The method of claim 1 , wherein said detector comprises a probe, and said gaseous organic compound is detected via the probe selectively reacting with said gaseous organic compound.

9. A method for detecting an analyte in a bodily liquid fluid, comprising:
placing a dressing in contact with a human or animal skin, said skin excreting said bodily liquid fluid, said bodily liquid fluid including said analyte, said dressing including at least a detector, said detector being located above the human or animal skin,
degrading or metabolizing said analyte to release a Volatile Organic Compound (VOC), said VOC being in a liquid form; and
detecting a gaseous form of the VOC with said detector, when said liquid form of the VOC evaporates into said gaseous form of the VOC, wherein
said detector is an optical detector, where the optical detector is configured such that the presence of a gaseous form of the VOC modifies optical properties of the optical detector, and
said detector does not contact said bodily liquid fluid.

10. The method of claim 1 , wherein the detector includes a probe, where the probe is specific
to a predetermined Volatile Organic Compound released by the analyte, or
to a predetermined class of Volatile Organic Compounds released by the analyte, and

the probe is incorporated into the support by trapping, by adsorption or by covalent bonding.

11. The method of claim 10 , wherein the probe has one or more functions configured to interact with the predetermined Volatile Organic Compound released by the analyte such that, once bound to the predetermined Volatile Organic Compound released by the analyte, the probe produces an optically detectable signal.

12. The method of claim 11 , wherein the detection of said gaseous organic compound with said detector comprises forming an absorbing or fluorescent compound by reacting the probe with the gaseous form of the VOC.

13. The method of claim 1 , wherein the VOC in a liquid form that is released by the analyte is selected from the group consisting of ethanol, acetone, acetaldehyde, acetic acid, dimethyl sulfide, methane thiol, indole, aminoacetophenone, trimethylamine, hexanal, methanol, putrescine, cadaverine, CH 3 SH and cyclohexanone.

14. The method of claim 1 , wherein said detector includes a probe selected from the group consisting of 4-aminopent-3-in-2-one, dimethylaminobenzaldehyde, 4-dimethylaminocinnamaldehyde, cyclohexanone and acetaldehyde.

15. The method of claim 1 , wherein
said detector includes a probe comprising dimethylaminobenzaldehyde, and the gaseous organic compound is an indole;
said detector includes a probe comprising 4-dimethylaminocinnamaldehyde, and the gaseous organic compound is an indole;
said detector includes a probe comprising 4-aminopent-3-in-2-one, and the gaseous organic compound is an aldehyde;
said detector includes a probe comprising cyclohexanone, and the gaseous organic compound is 2′aminoacétophénone; or
said detector includes a probe comprising an aldehyde, and the gaseous organic compound is 2′aminoacétophénone.

Assignees

• Marchand Gilles
• Marcoux Pierre
• Commissariat à l'Energie Atomique et aux Energies Alternatives

Inventors

• Marchand Gilles
• Marcoux Pierre

Classifications

• A61F2013/00846

  with transparent or translucent part · CPC title

• A61B5/00

  Measuring for diagnostic purposes (radiation diagnosis A61B6/00; diagnosis by ultrasonic, sonic or infrasonic waves A61B8/00); Identification of persons · CPC title

• A61F13/0213

  the fluid retention member being a layer of hydrocolloid, gel forming material · CPC title

• G01N33/5088 Primary

  of vertebrates · CPC title

• A61F13/0273

  Adhesive bandages for winding around limb, trunk or head, e.g. cohesive · CPC title