Micro titre plate

Patent metadata

Field	Value
Publication number	US-9551694-B2
Application number	US-200913122585-A
Country	US
Kind code	B2
Filing date	Oct 7, 2009
Priority date	Oct 15, 2008
Publication date	Jan 24, 2017
Grant date	Jan 24, 2017

Abstract

A micro titer plate comprising a number of wells filled with separation matrix. According to the invention the volume of the separation matrix is varied between at least some of the wells.

First claim

What is claimed is:

1. A micro titre plate comprising a number of wells disposed in a plurality of columns and rows, the wells being filled with the same type of separation matrix having a series of volumes V 1 , V 2 , . . . V n distributed according to a specific pattern of FIG. 1, 2 , or 3 and FIG. 4 .

2. The micro titre plate of claim 1 wherein the series of volumes V 1 , V 2 , . . . V n is distributed according to a specific pattern for the calculation of an adsorption isotherm of the separation matrix such that the series of volumes results in a series of corresponding isotherm data points in the calculation of the adsorption isotherm.

3. The micro titre plate of claim 1 , wherein each well in the plate has a permeable filter in the bottom.

4. A method for determining an adsorption isotherm comprising the steps of:
using the micro titre plate of claim 1 ;
adding the same volume and concentration of a liquid sample to the number of wells needed for the analysis;
waiting for a predefined period of time until at least some of the sample is adsorbed onto the separation matrix; and
measuring concentration of the sample remaining in the liquid phase and/or solid phase in at least two wells with different volumes of separation matrix.

5. The method of claim 4 , wherein the predefined waiting time is until an equilibrium between the liquid and solid phases has been reached.

6. A method for determining an adsorption isotherm comprising the steps of:
using a micro titre plate that comprises a number of wells disposed in a plurality of columns and rows, the wells being filled with the same type of separation matrix having a series of volumes V 1 , V 2 , . . . V n distributed according to a specific pattern of FIG. 1, 2 , or 3 and FIG. 4 ;
adding the same volume and concentration of a liquid sample to the number of wells needed for the analysis;
waiting for a predefined period of time until at least some of the sample is adsorbed onto the separation matrix; and
measuring concentration of the sample remaining in the liquid phase and/or solid phase in at least two wells with different volumes of separation matrix.

Assignees

• Lacki Karol
• Ge Healthcare Bioprocess R&D Ab

Inventors

• Lacki Karol

Classifications

• B01L3/5085

  for multiple samples, e.g. microtitration plates · CPC title

• G01N30/466

  with separation columns in parallel · CPC title

• B01L3/50255 Primary

  Multi-well filtration · CPC title

• G01N30/8658 Primary

  Optimising operation parameters · CPC title

• G01N30/6043

  in parallel · CPC title