Methods and compositions for selective regulation of protein expression
US-2024102042-A1 · Mar 28, 2024 · US
Cotton event pDAB4468.18.07.1 detection method
US9551024B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9551024-B2 |
| Application number | US-201313748516-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jan 23, 2013 |
| Priority date | Jan 23, 2012 |
| Publication date | Jan 24, 2017 |
| Grant date | Jan 24, 2017 |
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Abstract
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Cotton event pDAB4468.18.07.1 comprises gene expression cassettes which contain genes encoding aad-12 and pat, affording herbicide tolerance to cotton crops containing the event, and enabling methods for crop protection. Embodiments of the subject invention provide polynucleotide-related event detection methods.
First claim
Opening claim text (preview).
What is claimed is: 1. A method of detecting cotton event pDAB4468.18.07.1 in a sample comprising cotton DNA, said method comprising: contacting said sample with a first primer at least 10 bp in length that selectively binds to a flanking sequence within by 1-2886 of SEQ ID NO:1 or the complement thereof, and a second primer at least 10 bp in length that selectively binds to an insert sequence within by 2887-3206 of SEQ ID NO:1 or the complement thereof; and assaying for an amplicon generated between said primers, wherein the amplicon consists of a polynucleotide comprised within SEQ ID NO:1. 2. The method according to claim 1 , wherein said sample comprising cotton DNA is obtained from cotton plants comprising event pDAB4468.18.07.1 that have been deposited under ATCC accession number PTA-12456. 3. The method according to claim 1 , wherein the first primer comprises the polynucleotide of SEQ ID NO:5, and wherein the second primer comprises the polynucleotide of SEQ ID NO:6. 4. The method of claim 3 , wherein said sample comprising cotton DNA is obtained from cotton plants comprising event pDAB4468.18.07.1 that have been deposited under ATCC accession number PTA-12456.
Assignees
Inventors
Classifications
- C12N15/8274Primary
for herbicide resistance · CPC title
for plants, fungi or algae · CPC title
Phosphinotricin · CPC title
Primer sets for multiplex assays · CPC title
- C12Q1/6813Primary
Hybridisation assays · CPC title
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Frequently asked questions
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- What does patent US9551024B2 cover?
- Cotton event pDAB4468.18.07.1 comprises gene expression cassettes which contain genes encoding aad-12 and pat, affording herbicide tolerance to cotton crops containing the event, and enabling methods for crop protection. Embodiments of the subject invention provide polynucleotide-related event detection methods.
- Who is the assignee on this patent?
- Dow Agrosciences Llc
- What technology area does this patent fall under?
- Primary CPC classification C12N15/8274. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jan 24 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).