Regulation of epigenetic control of gene expression

Patent metadata

Field	Value
Publication number	US-9550990-B2
Application number	US-72132605-A
Country	US
Kind code	B2
Filing date	Dec 12, 2005
Priority date	Dec 10, 2004
Publication date	Jan 24, 2017
Grant date	Jan 24, 2017

Abstract

Methods are provided for the identification of compounds that selectively modulate epigenetic changes in gene expression. Compounds, compositions, kits or assays devices, and methods are provided for modulating the expression, endogenous levels or the function of small non-coding RNAs cognate to or transcribed by heterochromatic regions subject to epigenetic regulation (i.e., promoters, enhancers, centromeres, telomeres, origins of DNA replication, imprinted loci, or loci marked by dosage-compensation), and for modulating the formation or function of heterochromatin in cells, tissues or animals.

First claim

What is claimed is:

1. A method comprising:
identifying an oligomeric compound which modulates degradation or translational suppression of a target nucleic acid molecule;
identifying at least one heterochromatic region subject to epigenetic control of gene expression, wherein said heterochromatic region does not modulate expression of said target nucleic acid molecule; and
assaying for a modification to said heterochromatic region after treatment with said oligomeric compound.

2. The method of claim 1 , wherein said modification to said heterochromatic region is selected from the group consisting of acetylation, deacetylation, phosphorylation, dephosphorylation, methylation, demethylation, ubiquitination, sumoylation, pseudouridylation of said heterochromatic region and any combination thereof.

3. The method of claim 1 , wherein said modification to said heterochromatic region is a change in binding of at least one protein to said heterochromatic region.

4. The method of claim 1 , wherein the oligomeric compound is an siRNA.

Assignees

• Griffey Richard H
• Vickers Timothy
• Perera Ranjan
• Ionis Pharmaceuticals Inc

Inventors

• Griffey Richard H
• Vickers Timothy
• Perera Ranjan

Classifications

• C12N2310/346

  having a combination of backbone and sugar modifications · CPC title

• C12N2320/11

  for the determination of target sites, i.e. of active nucleic acids · CPC title

• C12N2310/341

  Gapmers, i.e. of the type ===---=== · CPC title

• Y10T436/143333

  Saccharide [e.g., DNA, etc.] · CPC title

• C12N2320/12

  in functional genomics, i.e. for the determination of gene function · CPC title