Micro-fluidic partitioning between polymeric sheets for chemical amplification and processing

Patent metadata

Field	Value
Publication number	US-9550186-B2
Application number	US-201514695968-A
Country	US
Kind code	B2
Filing date	Apr 24, 2015
Priority date	May 26, 2011
Publication date	Jan 24, 2017
Grant date	Jan 24, 2017

Abstract

A system for fluid partitioning for chemical amplification or other chemical processing or separations of a sample, comprising a first dispenser of a first polymeric sheet, wherein the first polymeric sheet contains chambers; a second dispenser of a second polymeric sheet wherein the first dispenser and the second dispenser are positioned so that the first polymeric sheet and the second polymeric sheet become parallel; a dispenser of the fluid positioned to dispense the fluid between the first polymeric sheet and the second polymeric sheet; and a seal unit that seals the first polymeric sheet and the second polymeric sheet together thereby sealing the sample between the first polymeric sheet and the second polymeric sheet and partitioning the fluid for chemical amplification or other chemical processing or separations.

First claim

The invention claimed is:

1. An apparatus for fluid partitioning for chemical amplification or other chemical processing or separations of a sample, comprising:
a first dispenser of a first polymeric sheet;
a second dispenser of a second polymeric sheet wherein said first dispenser and said second dispenser are positioned so that said first polymeric sheet and said second polymeric sheet become parallel;
a dispenser of the sample positioned to dispense the sample between said first polymeric sheet and said second polymeric sheet; and
a seal unit that seals said first polymeric sheet and said second polymeric sheet together thereby sealing the sample between said first polymeric sheet and said second polymeric sheet and partitioning the sample for chemical amplification or other chemical processing or separations, further comprising a chip for producing a magnetic field on said first polymeric sheet forming the sample into milli- to pico-liter-droplets oriented into a grid array.

2. The apparatus for fluid partitioning for chemical amplification or other chemical processing or separations of claim 1 further comprising a unit for coating said milli- to pico-liter-droplets with a monomer or dimer film.

3. The apparatus for fluid partitioning for chemical amplification or other chemical processing or separations of claim 2 further comprising a unit for curing said polymer film.

Assignees

• Anderson Brian L
• L Livermore Nat Security Llc

Inventors

• Anderson Brian L

Classifications

• Y10T156/1741

  Progressive continuous bonding press [e.g., roll couples] · CPC title

• B32B37/1027

  Pressing using at least one press band · CPC title

• C12Q1/6806 Primary

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title

• B65B9/023

  Packaging fluent material (B65B9/04 takes precedence) · CPC title

• B32B38/0008

  Electrical discharge treatment, e.g. corona, plasma treatment; wave energy or particle radiation (heat treatment B32B38/0036) · CPC title