Method and composition for inducing human pluripotent stem cells

Patent metadata

Field	Value
Publication number	US-9549954-B2
Application number	US-201214353284-A
Country	US
Kind code	B2
Filing date	Oct 22, 2012
Priority date	Oct 21, 2011
Publication date	Jan 24, 2017
Grant date	Jan 24, 2017

Abstract

The present invention provides a fibromodulin (FMOD) reprogrammed (FReP) cell and a method of making therefor, a culture medium therefor, and a supernatant thereof, and methods of making and using these.

First claim

We claim:

1. A fibromodulin (FMOD) reprogrammed (FReP) cell, generated by a method comprising:
treating a human cell with a cell culture medium for a period ranging from a day to a month, and
changing the cell culture medium regularly until the FReP cell forms;
wherein the medium comprises fibromodulin (FMOD),
the FReP cell expresses NANOG and does not form teratoma, and
wherein the human cell is a fibroblast cell.

2. The FReP cell according to claim 1 , wherein the FMOD has a concentration from about 200 nM to about 800 nM.

3. The FReP cell according to claim 1 , wherein the human cell is a BJ fibroblast or primary adult normal human dermal fibroblast (NHDF).

4. A composition comprising the FReP cell of claim 1 .

5. The composition of claim 4 , which is a pharmaceutical or cosmetic composition.

Assignees

• Univ California

Inventors

• Soo B Chia
• Ting Kang
• Zheng Zhong

Classifications

• C07K14/435

  from animals; from humans · CPC title

• C12N5/0696 Primary

  Artificially induced pluripotent stem cells, e.g. iPS · CPC title

• A61K35/33 Primary

  Fibroblasts · CPC title

• C12N2501/15

  Transforming growth factor beta (TGF-β) · CPC title

• C12N5/06

  Animal cells or tissues; Human cells or tissues (preservation of excised living parts A01N1/10) · CPC title