Hemipteran-and coleopteran active toxin proteins from Bacillus thuringiensis

Patent metadata

Field	Value
Publication number	US-9546378-B2
Application number	US-201314107453-A
Country	US
Kind code	B2
Filing date	Dec 16, 2013
Priority date	Apr 27, 2007
Publication date	Jan 17, 2017
Grant date	Jan 17, 2017

Abstract

A novel Bacillus thuringiensis crystal protein exhibiting insect inhibitory activity is disclosed. Growth of Lygus insects is significantly inhibited by providing the novel crystal protein in Lygus insect diet. Polynucleotides encoding the crystal protein, transgenic plants and microorganisms that contain the polynucleotides, isolated peptides derived from the crystal protein, and antibodies directed against the crystal protein are also provided. Methods of using the crystal protein and polynucleotides encoding the crystal protein to control Hemipteran insects are also disclosed.

First claim

What is claimed is:

1. A method for controlling a pest infestation of a plant, said method comprising: providing in a diet of said pest a plant or part thereof, said plant or part thereof comprising an insect inhibitory protein encoded by a recombinant nucleic acid molecule comprising a heterologous promoter operably linked to a polynucleotide segment encoding an insect inhibitory protein, wherein the insect inhibitory protein comprises an amino acid sequence having at least 99% sequence identity to the amino acid sequence as set forth in SEQ ID NO:5.

2. The method of claim 1 , wherein said pest is selected from the group consisting of a Hemipteran insect, a Heteropteran insect, a Leptinotarsa sp. insect, a Homopteran insect, and a combination thereof.

3. The method of claim 1 , wherein said pest is selected from the group consisting of an aphid, a hopper, and a whitefly.

4. The method of claim 1 , wherein said pest is a Lygus insect.

5. The method of claim 1 , wherein said polynucleotide segment comprises a sequence selected from the group consisting of SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:50, SEQ ID NO:51, SEQ ID NO:52, and SEQ ID NO:53.

6. The method of claim 1 , wherein said plant or part thereof further comprises at least one insect inhibitory agent selected from the group consisting of ET29, ET37, TIC809, TIC810, TIC812, TIC127, TIC128, AXMI-027, AXMI-036, AXMI-038, AXMI-018, AXMI-020, AXMI-021, AXMI-010, AXMI-003, AXMI-008, AXMI-006, AXMI-007, AXMI-009, AXMI-014, AXMI-004, AXMI-028, AXMI-029, and AXMI-0080rf2.

Assignees

• Baum James A
• Flasinski Stanislaw
• Heck Gregory R
• Penn Stephen R
• Sukuru Uma Rao
• Shi Xiaohong
• Monsanto Technology Llc

Inventors

• Baum James A
• Flasinski Stanislaw
• Heck Gregory R
• Penn Stephen R
• Sukuru Uma Rao
• Shi Xiaohong

Classifications

• C12N15/8286 Primary

  for insect resistance · CPC title

• C07K14/325

  Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins · CPC title

• A01N63/00

  Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates (containing compounds of determined constitution A01N27/00 - A01N59/00; unicellular algae A01N65/03) · CPC title

• A01N63/02

  Human Necessities · mapped topic

• A01N63/50

  Isolated enzymes; Isolated proteins (peptides A01N37/46) · CPC title