CMV glycoproteins and recombinant vectors

Patent metadata

Field	Value
Publication number	US-9541553-B2
Application number	US-201414179152-A
Country	US
Kind code	B2
Filing date	Feb 12, 2014
Priority date	Mar 25, 2010
Publication date	Jan 10, 2017
Grant date	Jan 10, 2017

Abstract

This invention also relates to recombinant vectors expressing one or more of the human CMV (HCMV) glycoproteins US2, US3, US6 and US11 or corresponding functional rhesus CMV (RhCMV) homologues Rh182, Rh184, Rh185 or Rh189, methods of making them, uses for them, expression products from them, and uses for the expression products. This invention also relates to recombinant cytomegalovirus vectors vectors lacking one or more of the glycoproteins, methods of making them, uses for them, expression products from them, and uses for the expression products.

First claim

What is claimed is:

1. A method of determining efficacy of a cytomegalovirus (CMV) immunogenic composition, comprising the steps of:
(a) administering the immunogenic composition to a CMV seronegative test subject, wherein the immunogenic composition comprises or expresses at least one CMV immunogen and elicits a CD8+ T cell response to the at least one CMV immunogen within the test subject;
(b) detecting the presence of a pre-challenge CD8+ T cell response of the test subject to the at least one CMV immunogen;
(c) challenging the test subject with a recombinant CMV challenge virus expressing the at least one CMV immunogen, wherein the challenge virus is capable of eliciting a CD8+ T cell response to the at least one CMV immunogen within a CMV seronegative host; and
(d) detecting the presence or absence of a post-challenge CD8+ T cell response of the test subject to the at least one CMV immunogen, wherein the immunogenic composition is efficacious in eliciting a protective immune response against CMV if a post-challenge boost to the CD8+ T cell response of the test subject to the at least one CMV immunogen is absent; and
wherein:
(i) (1) the CMV seronegative test subject is a human CMV (HCMV) seronegative test subject, (2) the at least one CMV immunogen is a HCMV immunogen, (3) the immunogenic composition does not comprise a recombinant HCMV virus lacking the glycoproteins US2, US3, US6, and US11, (4) the recombinant CMV challenge virus is a recombinant HCMV challenge virus, and (5) the glycoproteins US2, US3, US6, and US11 are deleted in the challenge virus; or
(ii) (1) the CMV seronegative test subject is a rhesus CMV (RhCMV) seronegative test subject, (2) the at least one CMV immunogen is a RhCMV immunogen, (3) the immunogenic composition does not comprise a recombinant RhCMV virus lacking the glycoproteins Rh182, Rh184, Rh185, and Rh189, (4) the recombinant CMV challenge virus is a recombinant RhCMV challenge virus, and (5) the glycoproteins Rh182, Rh184, Rh185, and Rh189 are deleted in the challenge virus.

2. The method of claim 1 , wherein: (1) the CMV seronegative test subject is a HCMV seronegative test subject, (2) the at least one CMV immunogen is a HCMV immunogen, (3) the immunogenic composition does not comprise a recombinant HCMV virus lacking the glycoproteins US2, US3, US6, and US11, (4) the recombinant CMV challenge virus is a recombinant HCMV challenge virus, and (5) the glycoproteins US2, US3, US6, and US11 are deleted in the challenge virus.

3. The method of claim 2 , wherein the immunogenic composition comprises an attenuated strain of HCMV.

4. The method of claim 3 , wherein the immunogenic composition lacks the transactivator pp71.

5. The method of claim 2 , wherein the immunogenic composition comprises a non-attenuated strain of HCMV.

6. The method of claim 2 , wherein all glycoproteins within the US2 to US11 region are deleted from the challenge virus.

7. The method of claim 1 , wherein: (1) the CMV seronegative test subject is a rhesus CMV (RhCMV) seronegative test subject, (2) the at least one CMV immunogen is a RhCMV immunogen, (3) the immunogenic composition does not comprise a recombinant RhCMV virus lacking the glycoproteins Rh182, Rh184, Rh185, and Rh189, (4) the recombinant CMV challenge virus is a recombinant RhCMV challenge virus, and (5) the glycoproteins Rh182, Rh184, Rh185, and Rh189 are deleted in the challenge virus.

8. The method of claim 7 , wherein the immunogenic composition comprises an attenuated strain of RhCMV.

9. The method of claim 8 , wherein the immunogenic composition lacks the transactivator pp71.

10. The method of claim 7 , wherein the immunogenic composition comprises a non-attenuated strain of RhCMV.

11. The method of claim 7 , wherein all glycoproteins within the Rh182-Rh189 region are deleted from the challenge virus.

12. A method of determining efficacy of a HCMV immunogenic composition, comprising the steps of:
(a) administering the immunogenic composition to a HCMV seronegative test subject, wherein the immunogenic composition (1) comprises or expresses at least one HCMV immunogen, (2) does not comprise or express at least one additional immunogen expressed by a recombinant HCMV challenge virus, and (3) does not comprise a recombinant HCMV virus lacking the glycoproteins US2, US3, US6, and US11;
(b) detecting the presence of a pre-challenge CD8+ T cell response of the test subject to the at least one HCMV immunogen and the absence of a pre-challenge CD8+ T cell response of the test subject to the at least one additional immunogen;
(c) challenging the test subject with the challenge virus expressing the at least one additional immunogen, wherein the glycoproteins US2, US3, US6, and US11 are deleted in the challenge virus, and wherein the challenge virus is capable of eliciting a CD8+ T cell response to the at least one additional immunogen within a HCMV seronegative host; and
(d) detecting the presence or absence of a post-challenge CD8+ T cell response of the test subject to the at least one additional immunogen, wherein the immunogenic composition is efficacious in eliciting a protective immune response against HCMV if a de novo post-challenge CD8+ T cell response of the test subject to the at least one additional immunogen is absent.

13. The method of claim 12 , wherein the at least one additional immunogen is a HCMV immunogen.

14. The method of claim 12 , wherein the immunogenic composition comprises an attenuated strain of HCMV.

15. The method of claim 14 , wherein the immunogenic composition lacks the transactivator pp71.

16. The method of claim 12 , wherein the immunogenic composition comprises a non-attenuated strain of HCMV.

17. The method of claim 12 , wherein all glycoproteins within the US2 to US11 region are deleted from the challenge virus.

18. A method of determining efficacy of a RhCMV immunogenic composition, comprising the steps of:
(a) administering the immunogenic composition to a RhCMV seronegative test subject, wherein the immunogenic composition (1) comprises or expresses at least one RhCMV immunogen, (2) does not comprise or express at least one additional immunogen expressed by a recombinant HCMV challenge virus, and (3) does not comprise a recombinant RhCMV virus lacking the glycoproteins Rh182, Rh184, Rh185, and Rh189;
(b) detecting the presence of a pre-challenge CD8+ T cell response of the test subject to the at least one HCMV immunogen and the absence of a pre-challenge CD8+ T cell response of the test subject to the at least one additional immunogen;
(c) challenging the test subject with the challenge virus expressing the at least one additional immunogen, wherein the glycoproteins Rh182, Rh184, Rh185, and Rh189 are deleted in the challenge virus, and wherein the challenge virus is capable of eliciting a CD8+ T cell response to the at least one additional immunogen within a RhCMV seronegative host; and
(d) detecting the presence or absence of a post-challenge CD8+ T cell response of the test subject to the at least one additional immunogen, wherein the immunogenic composition is efficacious in eliciting a protective immune response against RhCMV if a de novo post-challenge CD8+ T cell response of the test subject to the at least one additional immunogen is absent.

19. The method of claim 18 , wherein the at least one additional immunogen is a RhCMV immunogen.

20. The method of claim 18 , wherein the immunogenic composition comprises an attenuated strain of RhCMV.

21. The method of claim 20 , wherein the

Assignees

• Univ Oregon Health & Science
• Univ Oregon Health & Science

Inventors

• Picker Louis
• Frueh Klaus
• Hansen Scott G
• Powers Collin

Classifications

• A61K39/21 Primary

  Retroviridae, e.g. equine infectious anemia virus · CPC title

• C12N2710/16143

  viral genome or elements thereof as genetic vector · CPC title

• A61K39/00

  Medicinal preparations containing antigens or antibodies (materials for immunoassay G01N33/53) · CPC title

• C12N2710/16134

  Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein · CPC title

• A61K39/02

  Bacterial antigens · CPC title