Optical measuring apparatus and specimen discriminating and dispensing apparatus

Patent metadata

Field	Value
Publication number	US-9541489-B2
Application number	US-201113176781-A
Country	US
Kind code	B2
Filing date	Jul 6, 2011
Priority date	Jan 6, 2009
Publication date	Jan 10, 2017
Grant date	Jan 10, 2017

Abstract

The invention provides an optical measuring apparatus having a plurality of measuring sections each having a light irradiating section for irradiating light to specimens and a light receiving section for receiving optical data acquired by irradiating the light to the specimens and a flow rate calculating section for calculating flow rate values of the specimens based on a difference of measured times of the optical data measured by the plurality of measuring sections with respect to the specimens and a distance between the plurality of measuring sections. The optical measuring apparatus measures the optical data of the specimens by irradiating light to the specimens, i.e., the objects to be measured, dispersed within a sample fluid flowing through a flow passage. The optical measuring apparatus further includes a flow rate graph generating section for generating flow rate graph data in which the values of flow rate of the specimens calculated by the flow rate calculating section are arrayed in order of calculation in time-series manner and for outputting the generated flow rate graph data to a displaying section.

First claim

The invention claimed is:

1. An optical measuring apparatus for measuring optical data of specimens, which are objects to be measured, dispersed in a sample fluid flowing within a flow passage by irradiating light to said specimens, comprising:
a plurality of measuring sections each having a light irradiating section for irradiating light to said specimens and a light receiving section for receiving the optical data acquired by irradiating the light to said specimens;
a flow rate calculating section implemented by circuitry, the circuitry configured to calculate values of flow rate of said specimens based on a difference of measured times of said optical data measured by said plurality of measuring sections with respect to said specimens and a distance between said plurality of measuring sections; and
a flow rate graph generating section implemented by the circuitry, the circuitry configured to generate flow rate graph data in which the values of flow rate of said specimens calculated by said flow rate calculating section are arrayed in order of calculation in a time-series manner and outputting said generated flow rate graph data to a display section to display a flow rate graph,
wherein said flow rate graph generating section includes an area range specifying section implemented by the circuitry, the circuitry configured to specify a desired graph area range within said entire flow rate graph data of said previously generated flow rate graph, acquire the plurality of values of flow rate of said specimens only within said specified graph area range of flow rate, and exclude unnecessary values of flow rate outside of said specified graph area range of flow rate to drop a variation of the frequency of optical data of specimens.

2. The optical measuring apparatus according to claim 1 , wherein the values of flow rate of said specimens calculated by said flow rate calculating section are adopted as measurement parameters for judging flow conditions of said sample fluid.

3. The optical measuring apparatus according to claim 2 , further comprising a flow condition judging section implemented by the circuitry, the circuitry configured to judge the flow condition of said specimens within said flow passage based on said measurement parameters including the values of flow rate of said specimens and outputting the judged resultant data to an output section including said display section.

4. The optical measuring apparatus according to claim 1 , wherein said flow rate graph generating section generates flow rate graph data in which the values of flow rate of said specimens acquired by said area specifying section are arrayed in a time-series manner in order calculated by said flow rate calculating section; and
outputs said generated flow rate graph data to said display section.

5. A specimen discriminating and dispensing apparatus for sorting object specimens among specimens, that are objects to be measured, dispersed in a sample fluid flowing through a flow passage, comprising:
the optical measuring apparatus described in any one of claims 1 , 2 or 3 ; and
a dispensing section for dispensing said specimens discriminated based on said measurement parameters measured by said optical measuring apparatus to dispensing object regions through a nozzle.

6. The specimen discriminating and dispensing apparatus according to claim 5 , wherein said specimen discriminating and dispensing apparatus sorts said specimens corresponding to said flow rate graph data within said graph area range specified by said area specifying section of said optical measuring apparatus as said object specimens.

7. The specimen discriminating and dispensing apparatus according to claim 6 , wherein said specimen discriminating and dispensing apparatus dispenses the object specimens from a tip of said nozzle to said dispensing object region when the flow rate of said measured object specimen varies in sorting said specimens corresponding to said flow rate graph data within said graph area range specified by said area specifying section of said optical measuring apparatus, and
wherein the specimen discriminating and dispensing apparatus dispenses the object specimens by calculating a time for dispensing the object specimen based on a relationship related to the measured flow rate of the object specimen.

8. The specimen discriminating and dispensing apparatus according to claim 7 , wherein said specimen discriminating and dispensing apparatus dispenses one or a plurality of measured objects to said dispensing object regions after going through a flow rate slower than a flow rate at position where the specimen is measured.

9. The specimen discriminating and dispensing apparatus according to claim 8 , wherein said specimen discriminating and dispensing apparatus dispenses said object to be measured to said dispensing object region by switching a positional relationship between the tip of the nozzle of a pipe line and said dispensing object region by a time for dispensing said object to be measured calculated based on the measured and calculated flow rate.

10. The optical measuring apparatus according to claim 1 , wherein the plurality of values of flow rate of said specimens are acquired within said specified graph area range of flow rate independently of a calculated time period.

Assignees

• Tsukii Ken
• Takahashi Toru
• Xu Jie
• Furukawa Electric Co Ltd

Inventors

• Tsukii Ken
• Takahashi Toru
• Xu Jie

Classifications

• G01N15/14 Primary

  Optical investigation techniques, e.g. flow cytometry · CPC title

• G01F15/07

  Integration to give total flow, e.g. using mechanically-operated integrating mechanism · CPC title

• G01N2015/149

  Physics · mapped topic

• G01F1/7086

  using optical detecting arrangements · CPC title

• B07C5/344

  according to electric or electromagnetic properties {(sorting according to size measured electrically or electronically B07C5/08; material testing by magnetic means G01N24/00, G01N27/00, by electrical means G01N27/00; electrical measuring devices in general G01R; coin testing G07D5/00)} · CPC title