Stacked herbicide tolerance event 8291.45.36.2, related transgenic soybean lines, and detection thereof

Patent metadata

Field	Value
Publication number	US-9540656-B2
Application number	US-201113991309-A
Country	US
Kind code	B2
Filing date	Dec 2, 2011
Priority date	Dec 3, 2010
Publication date	Jan 10, 2017
Grant date	Jan 10, 2017

Abstract

This invention relates to soybean event pDAB8291.45.36.2, which includes a novel expression cassette comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding, and herbicide tolerant plants. In some embodiments, the event sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to detection methods, including endpoint TaqMan PCR assays, for the detection of Event pDAB8291.45.36.2 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.

First claim

The invention claimed is:

1. A transgenic soybean plant cell comprising a genome, said genome comprising SEQ ID NO: 27, wherein said SEQ ID NO: 27 comprises a first junction sequence represented by SEQ ID NO:16 and a second junction sequence represented by SEQ ID NO:17.

2. A soybean seed comprising a genome comprising Event pDAB8291.45.36.2 wherein said soybean seed is present in representative seed deposited with American Type Culture Collection (ATCC) under Accession No. PTA-11335.

3. A soybean seed comprising the transgenic soybean plant cell of claim 1 , said seed comprising said polynucleotide segment.

4. A soybean plant produced by growing the seed of claim 2 , said plant comprising said Event pDAB8291.45.36.2.

5. A progeny plant of the soybean plant of claim 4 , said progeny plant comprising said Event pDAB8291.45.36.2.

6. A transgenic soybean plant comprising a plurality of the transgenic soybean plant cells of claim 1 .

7. The transgenic soybean plant of claim 6 , said plurality of transgenic soybean plant cells further comprising an insect resistance gene.

8. The transgenic soybean plant of claim 6 wherein the plant is resistant to at least one herbicide selected from the group consisting of a phenoxyacetic acid herbicide, a phenoxybutanoic acid herbicide, a pyridyloxyalkanoic acid herbicide, a glyphosate herbicide, and a glufosinate herbicide.

9. A part of the soybean plant of claim 4 wherein said part is selected from the group consisting of pollen, an ovule, a flower, a shoot, a root, and a leaf, said part comprising said Event pDAB8291.45.36.2.

10. A soybean plant cell comprising a genome comprising Event pDAB8291.45.36.2 as present in representative seed deposited with American Type Culture Collection (ATCC) under Accession No. PTA-11335.

11. An isolated polynucleotide wherein said polynucleotide comprises a nucleotide sequence selected from the group consisting of SEQ ID NOS: 13, 14-17, and 27.

12. A method of controlling weeds in a field, said method comprising applying a herbicide selected from the group consisting of a phenoxyacetic acid, a phenoxybutanoic acid, a pyridyloxyalkanoic acid, a glyphosate, and a glufosinate to the field, and planting a seed of claim 3 in the field within 14 days of applying the herbicide.

13. The transgenic soybean plant of claim 6 , wherein the plant is from Glycine max.

14. A probe that is at least 95% identical to a full length sequence selected from the group consisting of SEQ ID NO: 14, SEQ ID NO: 15, and the complements thereof.

Assignees

• Cui Yunxing Cory
• Hoffman Thomas
• Zhou Ning
• Novak Stephen
• Colon Julissa
• Parkhurst Dawn M
• Toledo Sandra Grace
• Wright Terry R
• Russell Sean Michael
• Held Bruce
• Sekar Vaithilingam
• Dow Agrosciences Llc

Inventors

• Cui Yunxing Cory
• Hoffman Thomas
• Zhou Ning
• Novak Stephen
• Colon Julissa
• Parkhurst Dawn M
• Toledo Sandra Grace
• Wright Terry R
• Russell Sean Michael
• Held Bruce
• Sekar Vaithilingam

Classifications

• C12N15/8275 Primary

  Glyphosate · CPC title

• C12N9/1029

  transferring groups other than amino-acyl groups (2.3.1) · CPC title

• C12N9/1092

  3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase · CPC title

• C12Y114/11017

  Taurine dioxygenase (1.14.11.17) · CPC title

• C12N15/82

  for plant cells {, e.g. plant artificial chromosomes (PACs)} · CPC title