Purification process

Patent metadata

Field	Value
Publication number	US-9540416-B2
Application number	US-201313828852-A
Country	US
Kind code	B2
Filing date	Mar 14, 2013
Priority date	Jul 27, 2012
Publication date	Jan 10, 2017
Grant date	Jan 10, 2017

Abstract

The present disclosure concerns methods for producing purified saponins, such as QS-21, which reduces the number of necessary lyophilization cycles to one by a step of solvent exchange and optional subsequent exposure of the purified saponin product to at least one exposure to dry gas followed by exposure to a vacuum to further rid the purified saponin product of residual solvent molecules.

First claim

We claim:

1. A method for purifying at least one saponin in a solution comprising the steps of:
a) providing at least one saponin in a first solvent comprising a solubilizing component;
b) replacing at least a portion of the solubilizing component with an exchange solvent, thereby producing a replaced solvent; and
c) removing the replaced solvent, to produce a dried saponin product comprising at least one saponin, wherein the first solvent comprises at least partially purified QS-21; and the first solvent comprises at least 22% volume/volume acetonitrile; and the replaced solvent comprises no more than 21% volume/volume acetonitrile.

2. The method of claim 1 , wherein the solubilizing component of step (a) completely or partially disperses the at least one saponin into the solvent.

3. The method of claim 1 , wherein the first solvent comprises an HPLC column eluate.

4. The method of claim 1 , wherein the first solvent comprises a C8 HPLC column eluate.

5. The method of claim 1 , wherein the first solvent comprises a phenyl chromatography column eluate.

6. The method of claim 1 , wherein the first solvent comprises at least about 50% volume/volume acetonitrile.

7. The method of claim 1 , wherein the first solvent comprises between about 30% and 65% volume/volume acetonitrile.

8. The method of claim 1 , wherein the first solvent comprises between about 40% and 62% volume/volume acetonitrile.

9. The method of claim 1 , wherein the first solvent comprises between about 58% and 62% volume/volume acetonitrile.

10. The method of claim 1 , wherein replacing the solubilizing component is by one or more of: diafiltration, ultrafiltration or dialysis.

11. The method of claim 1 , wherein exchanging the at least a portion of the solubilizing component is by exchanging an identical volume of the first solvent with an exchange solvent.

12. The method of claim 1 , wherein replacing the at least a portion of the solubilizing component is by exchanging a non-identical volume of the first solvent with an exchange solvent.

13. The method of claim 1 , wherein the replaced solvent comprises at least 15% volume/volume acetonitrile.

14. The method of claim 1 , wherein the replaced solvent comprises between 15% and 21% volume/volume acetonitrile.

15. The method of claim 1 , wherein replacing the at least a portion of the solubilizing component is done in a system comprising a semi-permeable containment vessel.

16. The method of claim 15 , wherein the semi-permeable containment vessel is selectively permeable such that when the at least one solvent component will pass through the permeable portion of the vessel and at least one saponin will be retained.

17. The method of claim 15 , wherein the semi-permeable containment vessel comprises a single semi-permeable membrane.

18. The method of claim 17 , wherein solvent replacement is achieved by immersing the semi-permeable containment vessel comprising the at least one saponin and a first solvent in an exchange solvent and allowing the solvents separated by the membrane to reach equilibrium by diffusion.

19. The method of claim 15 , wherein the semi-permeable containment vessel comprises a channel that is surrounded by a semi-permeable structure.

20. The method of claim 19 , wherein the channel is entirely permeable to the solution including the at least one saponin in a solvent.

21. The method of claim 19 , wherein the channel constitutes the inside of the containment vessel, and any portion of the semi-permeable structure not in contact with the channel constitutes the outside of the containment vessel.

22. The method of claim 21 comprising moving the at least one saponin and a first solvent through the channel of the containment vessel at a positive pressure relative to the outside of the vessel such that at least one component of the solution is forced across the semi-permeable portion of the containment vessel.

23. The method of claim 22 , wherein replacing at least a portion of the solubilizing component with an exchange solvent is done by adding the exchange solvent to the inside of the containment vessel.

24. The method of claim 15 , wherein the semi-permeable containment vessel comprises a non-permeable container comprising a first compartment and a second compartment separated by a semi-permeable membrane.

25. The method of claim 24 , further comprising placing the at least one saponin and a first solvent into the first compartment of the containment vessel.

26. The method of claim 25 further comprising making the first compartment of the containment vessel a positive pressure relative to the second compartment such that at least one component of the solution is forced across the semi-permeable portion of the containment vessel.

27. The method of claim 26 further comprising replacing at least a portion of the solubilizing component with an exchange solvent by adding exchange solvent to the first compartment of the vessel.

28. The method of claim 1 , further comprising removing the replaced solvent by one or more of: lyophilization, heat exposure or rotary evaporation.

29. The method of claim 1 , further comprising repeating the removal step at least once.

Assignees

• Diaz Garcia Juan Jose
• Holzer Margit
• Glaxosmithkline Biologicals Sa

Inventors

• Diaz Garcia Juan Jose
• Holzer Margit

Classifications

• A61P37/04

  Immunostimulants · CPC title

• C07H1/08 Primary

  from natural products · CPC title

• C07H15/256

  Polyterpene radicals · CPC title

• C07J71/0052 Primary

  at position 16(17) · CPC title

• A61K2236/00

  Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine · CPC title