Optimizing virtual slide image quality

Patent metadata

Field	Value
Publication number	US-9535243-B2
Application number	US-201414310260-A
Country	US
Kind code	B2
Filing date	Jun 20, 2014
Priority date	May 3, 2000
Publication date	Jan 3, 2017
Grant date	Jan 3, 2017

Abstract

Systems, methods, and media for assessing the quality of a microscope slide image. In an embodiment, a plurality of focus point values are acquired for a sample on a microscope slide. Each focus point value comprises x-y coordinates indicating a location on the sample and a z coordinate indicating a focus height for the location on the sample. A best-fit surface is calculated based on the focus point values, and it is determined whether or not outlying focus point values exist based on the best-fit surface and the z coordinate for one or more of the focus point values. A scanned image of the microscope slide may be displayed which comprises, for each outlying focus point value, an overlay that identifies the location on the sample of the outlying focus point value based on the x-y coordinates for the outlying focus point value.

First claim

What is claimed is:

1. A non-transitory computer-readable medium having instructions stored thereon for assessing a quality of a microscope slide image, wherein the instructions, when executed by a processor, cause the processor to:
acquire a plurality of focus point values for a sample on a microscope slide, wherein each of the plurality of focus point values comprises x-y coordinates indicating a location on the sample and a z coordinate indicating a focus height for the location on the sample;
calculate a best-fit surface based on the plurality of focus point values;
determine whether or not the plurality of focus point values comprise outlying focus point values, based on the best-fit surface and the z coordinate for one or more of the plurality of focus point values, by, at least in part, for each of the plurality of focus point values,
removing a tilt from the best-fit surface and adjusting the focus point value,
determining a distance between the adjusted focus point value and the untilted best-fit surface, and,
if the distance is greater than a threshold value, determining that the focus point value is an outlying focus point value; and

display a scanned image of the microscope slide within a graphical display, wherein the scanned image of the microscope slide comprises, for each of the outlying focus point values, an overlay that identifies the location on the sample of the outlying focus point value based on the x-y coordinates for the outlying focus point value, and wherein the graphical display provides access to a re-scan mode; and,
when a user initiates the re-scan mode via the graphical display, initiate rescanning of the microscope slide.

2. The non-transitory computer-readable medium of claim 1 , wherein the instructions further cause the processor to initiate scanning of the microscope slide to acquire the scanned image of the microscope slide.

3. The non-transitory computer-readable medium of claim 2 , wherein the instructions further cause the processor to determine a focal surface based on the plurality of focus point values, and wherein the scanning of the microscope slide is performed in accordance with the focal surface.

4. The non-transitory computer-readable medium of claim 1 , wherein each overlay comprises an outline surrounding the location on the sample of the outlying focus point value.

5. The non-transitory computer-readable medium of claim 1 , wherein the best-fit surface is a substantially planar surface that represents an average focus height of the plurality of focus point values.

6. The non-transitory computer-readable medium of claim 1 , wherein calculating a best-fit surface based on the plurality of focus point values comprises, based on the plurality of focus point values, determining a least-squares solution for a plurality of parameters in a surface equation.

7. A system for assessing a quality of a microscope slide image, the system comprising:
at least one hardware processor; and
one or more modules that, when executed by the at least one hardware processor,
acquire a plurality of focus point values for a sample on a microscope slide, wherein each of the plurality of focus point values comprises x-y coordinates indicating a location on the sample and a z coordinate indicating a focus height for the location on the sample,
calculate a best-fit surface based on the plurality of focus point values,
determine whether or not the plurality of focus point values comprise outlying focus point values, based on the best-fit surface and the z coordinate for one or more of the plurality of focus point values, by, at least in part, for each of the plurality of focus point values,
removing a tilt from the best-fit surface and adjusting the focus point value,
determining a distance between the adjusted focus point value and the untilted best-fit surface, and,
if the distance is greater than a threshold value, determining that the focus point value is an outlying focus point value, and

display a scanned image of the microscope slide within a graphical display, wherein the scanned image of the microscope slide comprises, for each of the outlying focus point values, an overlay that identifies the location on the sample of the outlying focus point value based on the x-y coordinates for the outlying focus point value, and wherein the graphical display provides access to a re-scan mode, and,
when a user initiates the re-scan mode via the graphical display, initiate rescanning of the microscope slide.

8. The system of claim 7 , wherein the one or more modules further initiate scanning of the microscope slide to acquire the scanned image of the microscope slide.

9. The system of claim 8 , wherein the one or more modules further determine a focal surface based on the plurality of focus point values, and wherein the scanning of the microscope slide is performed in accordance with the focal surface.

10. The system of claim 7 , wherein each overlay comprises an outline surrounding the location on the sample of the outlying focus point value.

11. The system of claim 7 , wherein the best-fit surface is a substantially planar surface that represents an average focus height of the plurality of focus point values.

12. The system of claim 7 , wherein calculating a best-fit surface based on the plurality of focus point values comprises, based on the plurality of focus point values, determining a least-squares solution for a plurality of parameters in a surface equation.

13. A method for assessing a quality of a microscope slide image, the method comprising using at least one hardware processor to:
acquire a plurality of focus point values for a sample on a microscope slide, wherein each of the plurality of focus point values comprises x-y coordinates indicating a location on the sample and a z coordinate indicating a focus height for the location on the sample;
calculate a best-fit surface based on the plurality of focus point values;
determine whether or not the plurality of focus point values comprise outlying focus point values, based on the best-fit surface and the z coordinate for one or more of the plurality of focus point values, by, at least in part, for each of the plurality of focus point values,
removing a tilt from the best-fit surface and adjusting the focus point value,
determining a distance between the adjusted focus point value and the untilted best-fit surface, and,
if the distance is greater than a threshold value, determining that the focus point value is an outlying focus point value; and

display a scanned image of the microscope slide within a graphical display, wherein the scanned image of the microscope slide comprises, for each of the outlying focus point values, an overlay that identifies the location on the sample of the outlying focus point value based on the x-y coordinates for the outlying focus point value, and wherein the graphical display provides access to a re-scan mode; and,
when a user initiates the re-scan mode via the graphical display, initiate rescanning of the microscope slide.

14. The method of claim 13 , further comprising using the at least one hardware processor to initiate scanning of the microscope slide to acquire the scanned image of the microscope slide.

15. The method of claim 14 , further comprising using the at least one hardware processor to determine a focal surface based on the plurality of focus point values, wherein the scanning of the microscope slide is performed in accordance with the focal surface.

16. The method of claim 13 , wherein each overlay comprises an outline surrounding the location on t

Assignees

• Leica Biosystems Imaging Inc

Inventors

• Olson Allen
• Soenksen Dirk G
• Saligrama Kiran

Classifications

• G02B21/245 Primary

  using auxiliary sources, detectors · CPC title

• G02B21/002 Primary

  Scanning microscopes (scanning near field optical microscopes G01Q60/18) · CPC title

• H04N23/67

  Focus control based on electronic image sensor signals · CPC title

• G02B21/365

  Control or image processing arrangements for digital or video microscopes (G02B21/361, G02B21/362 take precedence) · CPC title

• G02B21/244

  using image analysis techniques · CPC title