Method and device for controlling the fermentation process

Patent metadata

Field	Value
Publication number	US-9534245-B2
Application number	US-98937508-A
Country	US
Kind code	B2
Filing date	Apr 23, 2008
Priority date	Apr 23, 2008
Publication date	Jan 3, 2017
Grant date	Jan 3, 2017

Abstract

A method and device for controlling a fermenting process, wherein the method comprises collecting samples of biological cells from a fermenting tank, obtaining the state information of the current biological cells based on the collected samples, comparing the state information of the current biological cells with preset target status information to obtain the difference between the state information of the current biological cells and preset target state information, and controlling the feed rate of nutritional solution into the fermenting tank. Real time control of biological fermenting process is thus accomplished based on the state information of the biological cells during fermentation, and the consistency during fermentation is improved. Complicated mathematical modeling and man-made data analysis are not required for the method and device. Real time control of biological fermenting process is accomplished based on a feedback loop with simply calculation, and control delay is not created, and automatic control of cells statement in fermentation is achieved.

First claim

The invention claimed is:

1. A device for controlling a fermentation process, comprising:
a status information acquisition unit configured to collect biological cell samples of biological cells from a fermenting tank and to obtain current status information of the biological cells based on the collected biological cell samples;
a control unit configured to control a feed rate of nutrient solution flow to said fermenting tank according to a difference status between the current status information of the biological cells and the preset target status information; and
a comparison unit configured to compare said current status information of the biological cells with a preset target status information, obtain the difference status between the current status information of the biological cells and the preset target status information, and to provide the difference status to said control unit.

2. The device as claimed in claim 1 , wherein said status information acquisition unit is a flow cytometer comprising:
a collection subunit configured to collect the biological cell samples from the fermenting tank and inject the collected biological cell samples into a center of sheath fluid in a flow chamber configured to allow collected biological cell samples to align in a single line and form a cell bundle under restriction of the sheath fluid;
a laser generator configured to generate a laser and excite said cell bundle to produce light information; and
a detector configured to obtain the current status information of the biological cells from said light information.

3. The device as claimed in claim 1 , wherein the device further comprises:
a target status information storage unit for storing the target status information, the comparison unit being further configured to obtain said target status information from said target status information storage unit.

4. The device as claimed in claim 1 , wherein said control unit comprises:
a feeding operation subunit configured to feed the nutrient solution to said fermenting tank based on the determined feed rate of nutrient solution flow;
a flow feed rate determination subunit configured to determine the feed rate of nutrient solution flow based on said difference status by using a preset flow feed rate determination methodology which causes the current status information of the biological cells in said fermenting tank to converge to said preset target status information, and to send the determined feed rate of nutrient solution flow to said feeding operation subunit.

5. The device as claimed in claim 4 , wherein said control unit further comprises:
a judgment subunit configured to receive the determined feed rate of nutrient solution flow sent from said flow feed rate determination subunit, and to judge whether the determined feed rate of nutrient solution flow is within a preset flow feed rate range;
wherein the judgment subunit sends the determined feed rate of nutrient solution flow to said feeding operation subunit if the feed rate of nutrient solution flow is within a preset flow feed rate range and sends a preset target flow feed rate of nutrient solution flow to said feeding operation subunit if the feed rate of nutrient solution flow is not within the preset flow feed rate range.

Assignees

• Bettenhausen Kurt Dirk
• Fan Shun Jie
• Grieb Herbert
• Jiang jun feng
• Yang Hongwei
• Zhuo Yue
• Siemens Ag

Inventors

• Bettenhausen Kurt Dirk
• Fan Shun Jie
• Grieb Herbert
• Jiang jun feng
• Yang Hongwei
• Zhuo Yue

Classifications

• G01N33/569

  for microorganisms, e.g. protozoa, bacteria, viruses · CPC title

• C12M41/46

  of cellular or enzymatic activity or functionality, e.g. cell viability · CPC title

• G01N33/5005

  involving human or animal cells (immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025) · CPC title

• C12Q1/02 Primary

  involving viable microorganisms · CPC title

• G01N33/56966

  Animal cells · CPC title