Polypeptides having xylanase activity and polynucleotides encoding same

Patent metadata

Field	Value
Publication number	US-9534211-B2
Application number	US-201013505891-A
Country	US
Kind code	B2
Filing date	Nov 5, 2010
Priority date	Nov 6, 2009
Publication date	Jan 3, 2017
Grant date	Jan 3, 2017

Abstract

The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

First claim

What is claimed is:

1. A nucleic acid construct or recombinant expression vector comprising an isolated polynucleotide encoding a polypeptide having xylanase activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct the production of the polypeptide in an expression host, and wherein the polypeptide having xylanase activity is selected from the group consisting of:
(a) a polypeptide comprising an amino acid sequence having at least 95% identity to the mature polypeptide of SEQ ID NO: 2; and
(b) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% identity to the mature polypeptide coding sequence of SEQ ID NO: 1.

2. The nucleic acid construct or recombinant expression vector of claim 1 , wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 2; or a fragment thereof having xylanase activity.

3. The nucleic acid construct or recombinant expression vector of claim 1 , wherein the polypeptide is encoded by the polynucleotide contained in plasmid pTF12Xyl170 which is contained in E. coli NRRL B-50309.

4. A recombinant host cell comprising the nucleic acid construct or recombinant expression vector of claim 1 .

5. A method of producing a polypeptide having xylanase activity, comprising: (a) cultivating the recombinant host cell of claim 4 under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.

6. A method of producing a polypeptide having xylanase activity, comprising:
(a) cultivating a transgenic plant or a plant cell comprising the nucleic acid construct or the expression vector of claim 1 under conditions conducive for production of the polypeptide; and
(b) recovering the polypeptide.

7. A transgenic plant, plant part or plant cell transformed with the nucleic acid construct or the expression vector of claim 1 .

8. A nucleic acid construct or recombinant expression vector comprising an isolated polynucleotide encoding a signal peptide comprising amino acids 1 to 19 of SEQ ID NO: 2 operably linked to a gene encoding a protein, wherein the gene is foreign to the polynucleotide encoding the signal peptide.

9. A method of producing a protein, comprising: (a) cultivating a recombinant host cell comprising a gene encoding a protein operably linked to a polynucleotide encoding a signal peptide comprising amino acids 1 to 19 of SEQ ID NO: 2, wherein the gene is foreign to the polynucleotide encoding the signal peptide, under conditions conducive for production of the protein; and (b) recovering the protein.

10. A method for degrading or converting a cellulosic material or xylan-containing material, comprising: treating the cellulosic material or xylan-containing material with an enzyme composition comprising a polypeptide having xylanase activity selected from the group consisting of:
(a) a polypeptide comprising an amino acid sequence having at least 95% identity to the mature polypeptide of SEQ ID NO: 2; and
(b) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% identity to the mature polypeptide coding sequence of SEQ ID NO: 1.

11. The method of claim 10 , further comprising recovering the degraded or converted cellulosic material or xylan-containing material.

12. A method for producing a fermentation product, comprising:
(a) saccharifying a cellulosic material or xylan-containing material with an enzyme composition comprising a polypeptide having xylanase activity selected from the group consisting of:
(i) a polypeptide comprising an amino acid sequence having at least 95% identity to the mature polypeptide of SEQ ID NO: 2; and
(ii) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% identity to the mature polypeptide coding sequence of SEQ ID NO: 1;
(b) fermenting the saccharified cellulosic material or xylan-containing material with one or more fermenting microorganisms to produce the fermentation product; and
(c) recovering the fermentation product from the fermentation.

13. A method of fermenting a cellulosic material or xylan-containing material, comprising: fermenting the cellulosic material or xylan-containing material with one or more fermenting microorganisms, wherein the cellulosic material or xylan-containing material is saccharified with an enzyme composition comprising a polypeptide having xylanase activity selected from the group consisting of:
(a) a polypeptide comprising an amino acid sequence having at least 95% identity to the mature polypeptide of SEQ ID NO: 2; and
(b) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% identity to the mature polypeptide coding sequence of SEQ ID NO: 1.

14. The method of claim 13 , wherein the fermenting of the cellulosic material or xylan-containing material produces a fermentation product.

15. The method of claim 14 , further comprising recovering the fermentation product from the fermentation.

16. The method of claim 14 , wherein the fermentation product is an alcohol, an organic acid, a ketone, an amino acid, or a gas.

17. The nucleic acid construct or recombinant expression vector of claim 1 , wherein the polypeptide comprises an amino acid sequence having at least 97% sequence identity to the mature polypeptide of SEQ ID NO: 2.

18. The nucleic acid construct or recombinant expression vector of claim 1 , wherein the polypeptide is encoded by a polynucleotide comprising a nucleotide sequence having at least 97% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1.

19. The method of claim 10 , wherein the polypeptide comprises an amino acid sequence having at least 97% sequence identity to the mature polypeptide of SEQ ID NO: 2.

20. The method of claim 10 , wherein the polypeptide is encoded by a polynucleotide comprising a nucleotide sequence having at least 97% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1.

21. The method of claim 12 , wherein the polypeptide comprises an amino acid sequence having at least 97% sequence identity to the mature polypeptide of SEQ ID NO: 2.

22. The method of claim 12 , wherein the polypeptide is encoded by a polynucleotide comprising a nucleotide sequence having at least 97% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1.

23. The method of claim 13 , wherein the polypeptide comprises an amino acid sequence having at least 97% sequence identity to the mature polypeptide of SEQ ID NO: 2.

24. The method of claim 13 , wherein the polypeptide is encoded by a polynucleotide comprising a nucleotide sequence having at least 97% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1.

25. The nucleic acid construct or recombinant expression vector of claim 1 , wherein the polypeptide comprises an amino acid sequence having at least 96% sequence identity to the mature polypeptide of SEQ ID NO: 2.

26. The nucleic acid construct or recombinant expression vector of claim 1 , wherein the polypeptide comprises an amino acid sequence having at least 98% sequence identity to the mature polypeptide of SEQ ID NO: 2.

27. The nucleic acid construct or recombinant expression vector of claim 1 , wherein the polypeptide comprises an amino acid sequence having at least 99% sequence identity to the mature polypeptide of SEQ ID NO: 2

Assignees

• Vlasenko Elena
• Mcbrayer Brett
• Skovlund Dominique
• Landvik Sara
• Novozymes As
• Novozymes Inc

Inventors

• Vlasenko Elena
• Mcbrayer Brett
• Skovlund Dominique
• Landvik Sara

Classifications

• C12N9/2462

  Lysozyme (3.2.1.17) · CPC title

• C12N9/2434 Primary

  acting on beta-1,4-glucosidic bonds · CPC title

• Y02E50/16

  Cross-Sectional Technologies · mapped topic

• C12Y302/01008

  Endo-1,4-beta-xylanase (3.2.1.8) · CPC title

• C12P7/10

  substrate containing cellulosic material · CPC title