Methods and cellular structures
US-2024209323-A1 · Jun 27, 2024 · US
Culture of pluripotent autologous stem cells from oral mucosa
US9534201B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9534201-B2 |
| Application number | US-60578109-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 26, 2009 |
| Priority date | Apr 26, 2007 |
| Publication date | Jan 3, 2017 |
| Grant date | Jan 3, 2017 |
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- Title
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- Abstract
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Abstract
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The present invention provides a new readily accessible source of adult somatic stem cells from the gastrointestinal tract in general and oral mucosa in particular, methods for isolating pluripotent stem cells from oral mucosa, cells derived therefrom and uses thereof.
First claim
Opening claim text (preview).
What is claimed is: 1. A culture comprising pluripotent stem cells that originated in the lamina propria of the oral mucosa, in an in vitro culture medium that ensures growth in vitro without production of differentiated progeny cells at the expense of the pluripotent stem cells, wherein said stem cells each display all of the markers Nestin, p75, Sox2, Oct4 and Nanog. 2. The culture of claim 1 , wherein said culture medium is a low glucose medium. 3. The culture of claim 2 , wherein said low glucose medium is supplemented with 1-20% fetal calf serum and an antibiotic solution. 4. The culture of claim 1 , wherein said stem cells originated in the lamina propria of the gingival oral mucosa. 5. The culture of claim 1 , wherein said stem cells further express a plurality of the markers β-III tubulin, glial fibrillar acid protein (GFAP), 04, Tra-1-61, Tral-81, SSEA1-4, Rex1, ABCG2, hTert, Bmil, CD29, CD44, CD73, CD90, CD105, CD106, CD117, CD146, CD166 and Strol+. 6. The culture of claim 1 , wherein said stem cells express the histocompatibility marker HLA-ABC. 7. The culture of claim 1 , wherein said stem cells constitutively express said markers when cultured in a low glucose medium supplemented with 10% serum and an antibiotic solution. 8. A pluripotent stem cell that originated in the lamina propria of the oral mucosa which displays all of the markers Nestin, p75, Sox2, Oct4 and Nanog, and which stem cell further includes a foreign gene that has been inserted into the stem cell by genetic manipulation.
Assignees
Inventors
Classifications
Cells of the oral mucosa · CPC title
- C12N5/0607Primary
Non-embryonic pluripotent stem cells, e.g. MASC (induced pluripotent stem cells C12N5/0696) · CPC title
Stomach; Intestine; Goblet cells; Oral mucosa; Saliva · CPC title
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Frequently asked questions
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- What does patent US9534201B2 cover?
- The present invention provides a new readily accessible source of adult somatic stem cells from the gastrointestinal tract in general and oral mucosa in particular, methods for isolating pluripotent stem cells from oral mucosa, cells derived therefrom and uses thereof.
- Who is the assignee on this patent?
- Pitaru Sandu, Ramot At Tel-Aviv Univ Ltd
- What technology area does this patent fall under?
- Primary CPC classification C12N5/0607. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jan 03 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).