Apparatus, system, and method for collecting a target material

Patent metadata

Field	Value
Publication number	US-9533303-B2
Application number	US-201514706606-A
Country	US
Kind code	B2
Filing date	May 7, 2015
Priority date	Nov 30, 2012
Publication date	Jan 3, 2017
Grant date	Jan 3, 2017

Abstract

This disclosure is directed to an apparatus, system and method for retrieving a target material from a suspension. A system includes a primary vessel, such as a tube, a collector, and a two-part seal including an internal pliant part and an external constricting part. The internal pliant part may be a float and the external constricting part may be a sealing ring. The system may also include a processing vessel, such as an Eppendorf tube, a syringe or a test tube. The collector is sized and shaped to fit into a primary vessel and funnels the target material from the suspension through a cannula and into the processing vessel. In one implementation, the processing vessel includes at least one displacement fluid to be expelled, such that the at least one displacement fluid pushes the target material into the collector.

First claim

We claim:

1. A system comprising:
a primary vessel comprising an open end, a suspension comprising a target material, and a float comprising a main body, wherein the float is located at a longitudinal position within the primary vessel;
a collector comprising an opening in a bottom end and a cavity in a top end connected by a cannula;
a processing vessel comprising
a first end comprising a plug,
an inner cavity, and
a displacement fluid having a density greater than a density of the target material, wherein the displacement fluid is located within the inner cavity; and

wherein a portion of an inner wall of the primary vessel is collapsed inwardly around a portion of the float,
wherein the bottom end of the collector is located within the open end of the primary vessel and the top end extends upwardly out of the open end of the primary vessel,
wherein at least a portion of the processing vessel is located within the cavity of the collector, and
wherein the cannula extends through the plug of the processing vessel.

2. The system of claim 1 , the main body of the float comprising at least one support member.

3. The system of claim 1 , the primary vessel further comprising at least one support member on an inner wall.

4. The system of claim 1 , wherein the displacement fluid is selected from the group consisting of: a solution of colloidal silica particles coated with polyvinylpyrrolidone, a polysaccharide solution, iodixanol, an organic solvent, a liquid wax, an oil, a gas, olive oil, mineral oil, silicone oil, immersion oil, mineral oil, paraffin oil, silicon oil, fluorosilicone, perfluorodecalin, perfluoroperhydrophenanthrene, perfluorooctylbromide, organic solvents, 1,4-Dioxane, acetonitrile, ethyl acetate, tert-butanol, cyclohexanone, methylene chloride, tert-Amyl alcohol, tert-Butyl methyl ether, butyl acetate, hexanol, nitrobenzene, toluene, octanol, octane, propylene carbonate, tetramethylene sulfones, ionic liquids, a polymer-based solution, a surfactant, a perfluoroketone, perfluorocyclopentanone, perfluorocyclohexanone, a fluorinated ketone, a hydrofluoroether, a hydrofluorocarbon, a perfluorocarbon, a perfluoropolyether, a silicon-based liquid, phenylmethyl siloxane, and combinations thereof.

5. The system of claim 1 , wherein the plug is resealable.

6. The system of claim 1 , wherein the collector further comprises a main body and a shoulder extending circumferentially around the main body.

7. The system of claim 6 , further comprising a lock ring placed over the shoulder of the collector and the open end of the primary vessel.

8. The system of claim 1 , further comprising a fluid-tight seal between the bottom end of the collector and an inner wall of the primary vessel.

Assignees

• Rarecyte Inc

Inventors

• Campton Daniel
• Nordberg Joshua
• Quarre Steve
• Stewart David
• Seubert Ronald
• Lundt Jonathan
• U'Ren Lance

Classifications

• B01L2200/026

  Fluid interfacing between devices or objects, e.g. connectors, inlet details · CPC title

• B01L2200/0631

  Purification arrangements, e.g. solid phase extraction [SPE] · CPC title

• B01L3/50215 Primary

  using a float to separate phases · CPC title

• B01L2300/0672

  Integrated piercing tool · CPC title

• B01L2400/0683

  mechanically breaking a wall or membrane within a channel or chamber · CPC title