Microvascular stamp for patterning of functional neovessels

Patent metadata

Field	Value
Publication number	US-9533073-B1
Application number	US-201514709726-A
Country	US
Kind code	B1
Filing date	May 12, 2015
Priority date	May 22, 2012
Publication date	Jan 3, 2017
Grant date	Jan 3, 2017

Abstract

The invention provides compositions and methods for making and using microvascular stamps for stimulation and spatial organization of neovessels in tissue.

First claim

We claim:

1. A method of inducing growth of new blood vessels in a tissue comprising contacting one or more microvascular stamps comprising one or more types of cells encapsulated in a poly(ethylene glycol) (PEGDA) and methacrylic polymer cross-linked hydrogel with the tissue, wherein the cross-linked hydrogel comprises microchannels, wherein the new blood vessels are localized within the lumina of the microchannels, and wherein the one or more microvascular stamps induce growth of new blood vessels in the tissue.

2. The method of claim 1 , wherein the one or more types of cells express one or more fusion proteins comprising a growth factor, proangiogenic factor, or antiangiogenic factor fused to a protein light switch.

3. The method of claim 2 , wherein the one or more microvascular stamps are illuminated with light at a wavelength of about 450 nm to about 500 nm.

4. The method of claim 1 , wherein microscale spacing between the new blood vessels is regulated by microchannel diameter and spacing.

5. The method of claim 1 , wherein the microscale spacing between the new blood vessels is about 100 μm to about 2.5 mm on-center.

6. The method of claim 1 , wherein the one or more types of cells secrete one or more proangiogenic factors, growth factors, or antiangiogenic factors.

7. The method of claim 1 , wherein the microchannels are about 100 μm to about 2.5 mm in diameter and wherein the on-center spacing between the microchannels is about 100 μm to about 2.5 mm.

8. The method of claim 1 , wherein the ratio between the flux of growth factors through a microchannel wall of the one or more microvascular stamps (J m ) is larger than the flux through a bottom of the one or more microvascular stamps with the same cross-sectional area as the microchannel (J r ).

9. The method of claim 1 , wherein the one or more microvascular stamps are about 100 μm to about 5 mm thick.

10. The method of claim 1 , wherein the one or more microvascular stamps have an elastic modulus of about 10 kPa to about 140 kPa.

11. The method of claim 1 , wherein the tissue is in vivo.

12. The method of claim 11 , wherein the tissue has a defect and the one or more microvascular stamps are contacted with the defect.

Assignees

• Univ Illinois

Inventors

• Kong Hyunjoon
• Bashir Rashid
• Jeong Jaehyun
• Chan Vincent
• Cha Chaenyung
• Zorlutuna Pinar

Classifications

• A61L2300/64

  Animal cells · CPC title

• A61L2430/00

  Materials or treatment for tissue regeneration · CPC title

• A61L27/52

  Hydrogels or hydrocolloids · CPC title

• A61L27/507 Primary

  for artificial blood vessels (apparatus for applying cells on a blood vessel prosthesis A61F2/062) · CPC title

• A61L27/26

  Mixtures of macromolecular compounds · CPC title