Apparatus for separating cells using magnetic force and cell separation method using the same
US-8951782-B2 · Feb 10, 2015 · US
US9532729B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9532729-B2 |
| Application number | US-201113825762-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 27, 2011 |
| Priority date | Sep 27, 2010 |
| Publication date | Jan 3, 2017 |
| Grant date | Jan 3, 2017 |
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A magnetic device includes a substrate and an array including a plurality of magnetic particles, in which a magnetic dipole moment direction of each magnetic particle in a majority of the magnetic particles in the array alternates with respect to a magnetic dipole direction of a directly adjacent magnetic particle or directly adjacent magnetic particles in the array.
Opening claim text (preview).
What is claimed is: 1. A magnetic device comprising: a substrate; an array including a plurality of magnetic particles arranged in a single layer on the substrate, wherein a magnetic dipole moment direction of each magnetic particle in a majority of the magnetic particles alternates with respect to a magnetic dipole direction of a directly adjacent magnetic particle in the array, wherein an average maximum dimension of each particle of the plurality of magnetic particles is between about 50 nm to about 100 microns, and wherein directly adjacent particles within the array are separated by a spacing of 5 nm or less; a fluid flow channel having a bottom surface arranged adjacent to the array, wherein the magnetic dipole moment direction of each magnetic particle in the majority of the magnetic particles is oriented orthogonal to the bottom surface of the fluid flow channel; and a chaotic mixer arranged in the fluid flow channel, wherein a structure of the chaotic mixer is arranged and configured to create a fluid force, within a fluid flowing in the fluid channel, in a direction toward the array. 2. The magnetic device according to claim 1 , wherein the magnetic particles in the array comprise permanent magnets. 3. The magnetic device according to claim 1 , wherein the magnetic particles in the array comprise a superparamagnetic material. 4. The magnetic device according to claim 1 , wherein the magnetic particles in the array comprise one or more of NdFeB, SmCo, FePt, and iron oxide. 5. The magnetic device according to claim 1 , further comprising a suspension layer, wherein the array of magnetic particles is located in the suspension layer. 6. The magnetic device according to claim 5 , wherein the suspension layer comprises a curable polymer. 7. The magnetic device according to claim 6 , wherein the polymer comprises polyurethane, epoxy, poly(methyl methacrylate) (PMMA), or polydimethylsiloxane (PDMS). 8. The magnetic device according to claim 1 , further comprising: a sheet coated with an adhesive that adheres to the array of magnetic particles. 9. The magnetic device according to claim 1 , wherein the plurality of magnetic particles in the array is close-packed. 10. The magnetic device according to claim 1 , further comprising a plurality of fluid flow channels arranged adjacent to the array.
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