DNA sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators
US-9115163-B2 · Aug 25, 2015 · US
US9528151B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9528151-B2 |
| Application number | US-201414242487-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 1, 2014 |
| Priority date | Dec 1, 2006 |
| Publication date | Dec 27, 2016 |
| Grant date | Dec 27, 2016 |
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This invention provides a process for sequencing single-stranded DNA employing modified nucleotides.
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What is claimed is: 1. A method, comprising: a) providing a plurality of primer strands bound to nucleic acid templates, a fraction of said plurality of primer strands comprising a free 3′-OH and another fraction of said plurality of primer strands comprising an incorporated labeled nucleotide analogue reversible terminator, wherein each reversible terminator comprises a cleavable moiety bound to the 3-oxygen of the deoxyribose, wherein a label is bound via a chemically cleavable…
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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