Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators

US9528151B2 · US · B2

Patent metadata
FieldValue
Publication numberUS-9528151-B2
Application numberUS-201414242487-A
CountryUS
Kind codeB2
Filing dateApr 1, 2014
Priority dateDec 1, 2006
Publication dateDec 27, 2016
Grant dateDec 27, 2016

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  2. Abstract

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  4. Key dates

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  5. First independent claim

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Abstract

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This invention provides a process for sequencing single-stranded DNA employing modified nucleotides.

First claim

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What is claimed is: 1. A method, comprising: a) providing a plurality of primer strands bound to nucleic acid templates, a fraction of said plurality of primer strands comprising a free 3′-OH and another fraction of said plurality of primer strands comprising an incorporated labeled nucleotide analogue reversible terminator, wherein each reversible terminator comprises a cleavable moiety bound to the 3-oxygen of the deoxyribose, wherein a label is bound via a chemically cleavable…

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What does patent US9528151B2 cover?
This invention provides a process for sequencing single-stranded DNA employing modified nucleotides.
Who is the assignee on this patent?
Univ Columbia
What technology area does this patent fall under?
Primary CPC classification C12Q1/6869. Mapped technology areas include Chemistry & Metallurgy.
When was this patent published?
Publication date Tue Dec 27 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
What related patents are in patentsdb?
We list 3 related publications on this page (citations in our corpus or others sharing the same primary CPC).