Quantitative pcr method using internal control
US-2024368681-A1 · Nov 7, 2024 · US
Method for analyzing multiple nucleic acid targets
US9528149B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9528149-B2 |
| Application number | US-201615049053-A |
| Country | US |
| Kind code | B2 |
| Filing date | Feb 20, 2016 |
| Priority date | Mar 10, 2015 |
| Publication date | Dec 27, 2016 |
| Grant date | Dec 27, 2016 |
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Abstract
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A detection device includes a PCR processor for conducting a PCR process on a first drop to a fourth drop flowing in a flow channel, a boundary detector for detecting intensities of fluorescence outputted from the first drop to the fourth drop after the PCR process and acquiring boundaries between the first drop to the fourth drop flowing in a flow channel based on the intensities of fluorescence, and a detector for acquiring a number of the second drop and the fourth drop having an intensity of fluorescence greater than or equal to a first threshold based on the intensity of fluorescence and boundaries between the first drop to the fourth drop, and detecting whether or not the objective nucleic acid target includes at least one selected from the group consisting of a first nucleic acid target and a second nucleic acid target based on the number of the second drop and the fourth drop.
First claim
Opening claim text (preview).
What is claimed is: 1. A method for detecting first nucleic acid targets and second nucleic acid targets using a microfluidic chip, the method comprising: (a) installing the microfluidic chip in a detection device, wherein the detection device comprises a PCR processor, a PCR reactor, a fluorescence detector, and a detect circuitry, the microfluidic chip comprises a first flow channel, a second flow channel, a third flow channel, a fourth flow channel, and a fifth flow channel…
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- C12Q1/686Primary
Chemistry & Metallurgy · mapped topic
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- What does patent US9528149B2 cover?
- A detection device includes a PCR processor for conducting a PCR process on a first drop to a fourth drop flowing in a flow channel, a boundary detector for detecting intensities of fluorescence outputted from the first drop to the fourth drop after the PCR process and acquiring boundaries between the first drop to the fourth drop flowing in a flow channel based on the intensities of fluorescen…
- Who is the assignee on this patent?
- Panasonic Ip Man Co Ltd
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/686. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Dec 27 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).