Rare earth spatial/spectral barcodes for multiplexed biochemical testing

Patent metadata

Field	Value
Publication number	US-9528145-B2
Application number	US-201414214594-A
Country	US
Kind code	B2
Filing date	Mar 14, 2014
Priority date	Mar 15, 2013
Publication date	Dec 27, 2016
Grant date	Dec 27, 2016

Abstract

Hydrogel microparticles spatially and spectrally encoded using upconverting phosphor nanoparticles are described for use in biochemical testing. In each microparticle, upconversion nanocrystals having spectrally distinguishable emission spectra are disposed in different pardons of an encoding region of the microparticle.

First claim

What is claimed:

1. A microparticle for use in a biochemical or chemical assay, the microparticle comprising:
a body comprising a hydrogel, the body including a probe region and an encoded region, the probe region including one or more molecular recognition elements;
a first plurality of upconversion nanocrystals disposed in a first portion of the encoded region, the first plurality of upconversion nanocrystals having a first spectral signature; and
a second plurality of upconversion nanocrystals disposed in a second portion of the encoded region the second plurality of upconversion nanocrystals having a second spectral signature spatially separated from the first portion of the encoded region.

2. The microparticle of claim 1 , wherein the second spectral signature is different than the first spectral signature.

3. The microparticle of claim 1 , wherein the first plurality of upconversion nanocrystals includes a first material doped with one or more rare earth elements and the second plurality of upconversion nanocrystals includes a second material doped with one or more rare earth elements.

4. The microparticle of claim 1 , wherein the body is asymmetric in shape.

5. The microparticle of claim 1 , wherein the upconversion nanocrystals are covalently bound to the hydrogel.

6. The microparticle of claim 1 , wherein the upconversion nanocrystals are bound to the hydrogel during particle synthesis through an acrylate group.

7. The microparticle of claim 1 , wherein for each portion of the encoded region, the plurality of upconversion nanocrystals are dispersed without aggregation.

8. The microparticle of claim 1 , further comprising a third plurality of upconversion nanocrystals disposed in a third portion of the encoded region spatially separated from the first portion of the encoded region and spatially separated from the second portion of the encoded region, the third plurality of upconversion nanocrystals having a third spectral signature different from or the same as any one of the first spectral signature or the second spectral signature.

9. The microparticle of claim 8 , wherein the encoded region includes at least five different portions.

10. The microparticle of claim 1 , wherein each spectral signature includes luminescence in multiple distinct bands within a range of 400-800 nm.

11. The microparticle of claim 1 , wherein the probe region comprises polyethylene glycol diacrylate (PEG-DA).

12. The microparticle of claim 1 , wherein the encoded region comprises di-acrylated monomer.

13. The microparticle of claim 1 , wherein the probe region comprises a first polymer material and the encoding region comprises a second polymer material different than the first polymer material.

14. The microparticle of claim 1 , wherein the upconversion nanocrystals are paramagnetic or ferromagnetic.

15. A method of making a hydrogel microparticle for use in a biochemical or chemical assay, the method comprising:
providing a first encoded region source material including a hydrogel and a first plurality of upconversion nanocrystals having a first spectral signature;
providing a second encoded region source material including a hydrogel and a second plurality of upconversion nanocrystals having a second spectral signature;
providing a probe region source material including a hydrogel and one or more molecular recognition elements;
cross-linking the first encoded region source material, the second encoded region source material and the probe region source material forming a first portion of an encoded region, a second portion of the encoded region spatially separated from the first portion, and a probe region with the probe region cross-linked with one or both of the first portion and the second portion of the encoded region to form a contiguous microparticle.

16. The method of claim 15 , wherein the second spectral signature is different than the first spectral signature.

17. The method of claim 15 , wherein each of the first plurality of upconversion nanocrystals and each of the second plurality of upconversion nanocrystals has a hydrophilic surface.

18. The method of claim 15 , wherein the method further comprises providing a third encoded region source material including a hydrogel and a third plurality of upconversion nanocrystals having a third spectral signature different from or the same as any one of the first spectral signature or the second spectral signature, and wherein the third encoded region source material forms a third portion of the encoded region of the microparticle spatially separated from the first portion of the encoded region and the second portion of the encoded region.

19. The method of claim 18 , wherein the method further comprises providing a fourth encoded region source material including a hydrogel and a fourth plurality of upconversion nanocrystals having a fourth spectral signature different from or the same as any one of the first spectral signature, the second spectral signature, or the third spectral signature, and wherein the fourth encoded region source material forms a fourth portion of the encoded region of the microparticle spatially separated from the first portion of the encoded region, the second portion of the encoded region, and the third portion of the encoded region.

20. The method of claim 15 , further comprising selecting the first plurality of upconversion nanocrystals and the second plurality of upconversion nanocrystals by comparing an expected first spectral response with an expected second spectral response, wherein the expected first spectral response is a convolution of an emission spectrum of the first plurality of upconversion nanocrystals and a spectral response of an image sensor, and wherein the expected second spectral response is a convolution of an emission spectrum of the second plurality of upconversion nanocrystals and a spectral response of an image sensor.

21. The method of claim 15 , wherein providing the first encoded region source material comprises forming the first plurality of upconversion nanocrystals.

22. The method of claim 15 , wherein providing the first encoded region source material comprises modifying a surface of each of the first plurality of upconversion nanocrystals.

23. The method of claim 15 , wherein the upconversion nanocrystals are paramagnetic or ferromagnetic.

24. A method of performing a biochemical or chemical assay comprising:
exposing a sample to a plurality of microparticles, each microparticle comprising:
a body comprising a hydrogel, the body including a probe region with one or more molecular recognition elements to detect a target of interest in the sample and an encoded region;
a first plurality of upconversion nanocrystals disposed in a first portion of the encoded region, the first plurality of upconversion nanocrystals having a first spectral signature; and
a second plurality of upconversion nanocrystals disposed in a second portion of the encoded region spatially separated from the first portion of the encoded region, the second plurality of upconversion nanocrystals having a second spectral signature different than the first spectral signature;

for each microparticle, illuminating the microparticle with an excitation light source;
for each microparticle, detecting light emitted from the illuminated microparticle, the detected light including upconverted luminescent light from the first plurality of upconversion nanocrystals and the second pl

Assignees

• Massachusetts Inst Technology

Inventors

• Bisso Paul
• Swiston Albert
• Lee Jiseok
• Doyle Patrick S

Classifications

• C09K11/7773 Primary

  with alkali or alkaline earth metal · CPC title

• Y10T436/143333

  Saccharide [e.g., DNA, etc.] · CPC title

• G07D7/2033

  Matching unique patterns, i.e. patterns that are unique to each individual paper · CPC title

• Y10T436/13

  Tracers or tags · CPC title

• G07D7/0043

  using barcodes · CPC title