Pseudomonas fluorescens2-79 with genes for biosynthesis of pyrrolnitrin improves biocontrol activity

Patent metadata

Field	Value
Publication number	US-9528115-B1
Application number	US-201414208543-A
Country	US
Kind code	B1
Filing date	Mar 13, 2014
Priority date	Mar 15, 2013
Publication date	Dec 27, 2016
Grant date	Dec 27, 2016

Abstract

In exemplary embodiments, the invention provides Pseudomonas fluorescens 2-79 strains having pyrrolnitrin biosynthetic genes for biocontrol of soilborne diseases.

First claim

What is claimed is:

1. A recombinant Pseudomonas fluorescens 2-79 strain comprising a stably transformed pyrrolnitrin (Prn) biosynthetic operon from Pseudomonas fluorescens Pf-5, wherein said recombinant Pseudomonas fluorescens 2-79 strain produces pyrrolnitrin from said Prn biosynthetic operon and wherein said recombinant Pseudomonas fluorescens 2-79 strain is a member selected from the group consisting of the recombinant Pseudomonas fluorescens 2-79 strains deposited under Reference Nos: NRRL 67312 and NRRL 67313.

2. The recombinant Pseudomonas fluorescens 2-79 strain of claim 1 , wherein said recombinant Pseudomonas fluorescens 2-79 strain has antibiotic activity against Rhizoctonia solani, Rhizoctonia oryzae, Gaeumannomyces graminis var, tritici , or a combination thereof.

3. The recombinant Pseudomonas fluorescens 2-79 strain of claim 2 , wherein said recombinant Pseudomonas fluorescens strain has enhanced antibiotic activity against Gaeumannomyces graminis var, tritici as compared to a parental Pseudomonas fluorescens 2-79 strain.

4. The recombinant Pseudomonas fluorescens 2-79 strain of claim 1 , wherein said recombinant Pseudomonas fluorescens 2-79 strain controls take-all and Rhizocotonia root rot significantly better than a parental Pseudomonas fluorescens 2-79 strain.

5. The recombinant Pseudomonas fluorescens 2-79 strain of claim 1 , wherein said recombinant Pseudomonas fluorescens 2-79 strain is the strain deposited under Reference No. NRRL 67312.

6. The recombinant Pseudomonas fluorescens 2-79 of claim 1 , wherein said recombinant Pseudomonas fluorescens 2-79 strain is the strain deposited under Reference No. NRRL 67313.

Assignees

• Us Agriculture
• Washington State Univ Res Found
• Univ Washington State

Inventors

• Weller David M
• Thomashow Linda S
• Mavrodi Dmitri V
• Yang Mingming
• Zhang Jibin

Classifications

• C12P17/10

  Nitrogen as only ring hetero atom · CPC title

• A01N43/36

  five-membered rings · CPC title

• C12N15/52 Primary

  Genes encoding for enzymes or proenzymes · CPC title

• C12N15/78 Primary

  for Pseudomonas · CPC title

• C12N2510/02

  Cells for production · CPC title