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Methods of efficiently establishing induced pluripotent stem cells under hypoxic conditions
US9528092B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9528092-B2 |
| Application number | US-67222209-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 30, 2009 |
| Priority date | Jul 30, 2008 |
| Publication date | Dec 27, 2016 |
| Grant date | Dec 27, 2016 |
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Abstract
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Provided is a method of improving the efficiency of establishment of induced pluripotent stem cells, comprising culturing somatic cells under hypoxic conditions in the step of nuclear reprogramming thereof.
First claim
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Then invention claimed is: 1. A method of improving the efficiency of establishment of mouse or human induced pluripotent stem (iPS) cells, comprising: a) introducing into mouse or human somatic cells an expression vector or expression vector expression vectors comprising nucleic acids encoding any of the following (i) to (ii): (i) Oct3/4, Klf4 and Sox2, (ii) Oct3/4, Klf4, Sox2 and c-Myc, and b) culturing the somatic cells from a) in ES cell culture medium, under hypoxic con…
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Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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- What does patent US9528092B2 cover?
- Provided is a method of improving the efficiency of establishment of induced pluripotent stem cells, comprising culturing somatic cells under hypoxic conditions in the step of nuclear reprogramming thereof.
- Who is the assignee on this patent?
- Yamanaka Shinya, Yoshida Yoshinori, Univ Kyoto
- What technology area does this patent fall under?
- Primary CPC classification C12N5/0696. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Dec 27 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).