Islet cell manufacturing compositions and methods of use
US-11945795-B2 · Apr 2, 2024 · US
US9528090B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9528090-B2 |
| Application number | US-201113211959-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 17, 2011 |
| Priority date | Aug 31, 2010 |
| Publication date | Dec 27, 2016 |
| Grant date | Dec 27, 2016 |
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The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method to produce a population of cells, wherein greater than 85% of the cells in the population express markers characteristic of the definitive endoderm lineage.
Opening claim text (preview).
What is claimed is: 1. An in vitro cell culture comprising an isolated population of cells and a culture medium suitable for differentiating pluripotent stem cells, wherein greater than 85% of the cells are definitive endoderm cells, wherein said population of cells is obtained by differentiating in vitro pluripotent stem cells, wherein the culture medium lacks serum and is supplemented with BSA, GDF-8, 14-Prop-2-en-1-yl-3,5,7,14,17,23,27-heptaazatetracyclo[19.3.1.1˜2,6˜0.1˜8,…
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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