Delivery of proteins using adeno-associated virus (AAV) vectors

Patent metadata

Field	Value
Publication number	US-9527904-B2
Application number	US-201414484842-A
Country	US
Kind code	B2
Filing date	Sep 12, 2014
Priority date	Feb 22, 2011
Publication date	Dec 27, 2016
Grant date	Dec 27, 2016

Abstract

Disclosed herein are compositions, systems and methods for delivery of proteins of interest using adeno-associated virus (AAV) vectors.

First claim

What is claimed is:

1. A method for producing a protein of interest in vivo, comprising:
providing a recombinant adeno-associated virus (AAV) comprising a promoter operably linked with a nucleotide sequence encoding the protein of interest, wherein the promoter comprises a nucleotide sequence having at least 95% sequence identity to the sequence of SEQ ID NO: 1; and
administering the recombinant AAV to a subject, whereby the recombinant AAV expresses the protein of interest in the subject.

2. The method of claim 1 , wherein the protein of interest is selected from the group consisting of an antibody, a growth hormone, an insulin-like growth factor, a G-CSF, an erythropoietin, an insulin, an antibody Fab fragment, an antibody scFV fragment, a hemophilia related clotting protein, a dystrophin, a lysosomal acid lipase, a phenylalanine hydroxylase, a glycogen storage disease-related enzyme, and any variant thereof.

3. The method of claim 1 , wherein the protein of interest is a full length antibody.

4. The method of claim 1 , wherein the protein of interest is selected from the group consisting of b12 anti-HIV antibody, 2G12 anti-HIV antibody, 4E10 anti-HIV antibody, 2F5 anti-HIV antibody, AR3A anti-HCV antibody, AR3B anti-HCV antibody, AR4A anti-HCV antibody, anti-malaria antibody, F10 anti-influenza antibody, FI6 anti-influenza antibody, TCN32 influenza antibody, CR6261 anti-influenza antibody, and any variant thereof.

5. The method of claim 2 , wherein the protein of interest is a virus neutralizing antibody or a neutralizing antibody for malaria.

6. The method of claim 1 , wherein the protein of interest is expressed in the serum of the subject in the amount of at least 100 μg/ml.

7. The method of claim 1 , wherein the recombinant AAV is produced by
providing a packaging cell line with a viral vector, helper functions for generating a productive AAV infection, and AAV cap genes, wherein the viral vector comprises a 5′ AAV inverted terminal repeat (ITR), a 3′ AAV ITR and a nucleotide sequence encoding the protein of interest and being operably linked with a promoter that comprises a nucleotide sequence having at least 95% sequence identity to the sequence of SEQ ID NO: 1; and
recovering a recombinant AAV virus from the supernatant of the packaging cell line.

8. A method for reducing or inhibiting the infection risk of a virus in a subject, comprising:
providing a recombinant adeno-associated virus (AAV) comprising a promoter operably linked with a nucleotide sequence encoding a neutralizing antibody for a virus, wherein the promoter comprises a nucleotide sequence having at least 95% sequence identity to the sequence of SEQ ID NO: 1; and
administering the recombinant AAV to a subject, whereby the recombinant AAV expresses the antibody in the subject, and whereby the infection risk of the virus in the subject is reduced or inhibited.

9. The method of claim 8 , wherein the method further comprises providing a second recombinant AAV comprising a nucleotide sequence encoding a second neutralizing antibody for the virus.

10. The method of claim 8 , wherein the subject is a mammal.

11. The method of claim 8 , wherein the subject is a human.

12. The method of claim 8 , wherein the neutralizing antibody is a full-length antibody.

13. The method of claim 8 , wherein the method reduces the infection risk in the subject by at least 5 folds as compared to the subject without the viral vector treatment.

14. The method of claim 8 , wherein the method inhibits the viral infection in the subject.

15. The method of claim 8 , wherein the virus is a human immunodeficiency virus (HIV), a hepatitis C virus (HCV), or an influenza virus.

16. The method of claim 8 , wherein the neutralizing antibody is selected from the group consisting of b12 anti-HIV antibody, 2G12 anti-HIV antibody, 4E10 anti-HIV antibody, 2F5 anti-HIV antibody, AR3A anti-HCV antibody, AR3B anti-HCV antibody, AR4A anti-HCV antibody, F10 anti-influenza antibody, FI6 anti-influenza antibody, TCN32 influenza antibody, CR6261 anti-influenza antibody, and any variant thereof.

17. The method of claim 8 , wherein the recombinant AAV is administered to the subject at most once every year.

18. The method of claim 1 , wherein the recombinant AAV is administered to the subject by intramuscular injection, intravaginal injection, intravenous injection, intraperitoneal injection, subcutaneous injection, epicutaneous administration, intradermal administration, or nasal administration.

19. The method of claim 1 , wherein the recombinant AAV expresses the protein of interest in the serum of the subject in the amount of at least 9 μg/ml.

20. The method of claim 1 , wherein the promoter comprises the nucleotide sequence of SEQ ID NO: 1.

21. The method of claim 8 , wherein the recombinant AAV is administered to the subject by intramuscular injection, intravaginal injection, intravenous injection, intraperitoneal injection, subcutaneous injection, epicutaneous administration, intradermal administration, or nasal administration.

22. The method of claim 8 , wherein the recombinant AAV expresses the protein of interest in the serum of the subject in the amount of at least 9 μg/ml.

23. The method of claim 8 , wherein the promoter comprises the nucleotide sequence of SEQ ID NO: 1.

Assignees

• California Inst Of Techn

Inventors

• Balazs Alejandro Benjamin
• Baltimore David

Classifications

• A61P31/12

  Antivirals · CPC title

• A61P31/18

  for HIV · CPC title

• C12N2750/14143

  viral genome or elements thereof as genetic vector · CPC title

• C12N7/00

  Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00) · CPC title

• C12N2750/14141

  Use of virus, viral particle or viral elements as a vector · CPC title