Cell culture assay

Patent metadata

Field	Value
Publication number	US-9523672-B2
Application number	US-201214354423-A
Country	US
Kind code	B2
Filing date	Oct 29, 2012
Priority date	Oct 28, 2011
Publication date	Dec 20, 2016
Grant date	Dec 20, 2016

Abstract

The present invention relates to a cell culture assay. The present invention comprises: a substrate; a scaffold channel which is formed along the center inside the substrate, and of which at least one is disposed continuously, and inside which a scaffold flows; and a microfluidic channel or channels which is/are respectively formed on one side or on both sides of the scaffold channel, and inside which cells flow, and, here, a leak-preventing part for preventing the scaffold from leaking into the microfluidic channel(s) is formed in at least one of the ceiling surface and the floor surface of a boundary part of the scaffold channel and the microfluidic channel(s).

First claim

What is claimed is:

1. A cell culture assay, comprising:
a substrate;
a scaffold channel which is formed along the centre inside the substrate, and of which at least one is disposed continuously, and inside which a scaffold flows; and
a microfluidic channel which is formed on one side or on both sides of the scaffold channel, and inside which cells flow, wherein at least one of the ceiling surface and the floor surface of a boundary part of the microfluidic channel and the scaffold channel includes a leak-preventing part for preventing the scaffold from leaking into the microfluidic channel, and
wherein the leak-preventing part is formed to account for 20% to 80% of the height of the scaffold channel and the microfluidic channel,
wherein the leak-preventing part is continuously disposed at a boundary part in which the scaffold channel meets the microfluidic channel in a longitudinal direction,
wherein the cell culture assay does not comprise a pole array to fix between scaffold channels.

2. The cell culture assay of claim 1 , wherein the microfluidic channel and the scaffold channel have semicircular upper parts, and the semicircular upper parts are in contact with each other, so that the leak-preventing part is formed at the boundary part therebetween.

3. The cell culture assay of claim 1 , wherein the leak-preventing part has a sharp tip having a cross section, the both sides of which are in the shape of a curve.

4. The cell culture assay of claim 1 , wherein the leak-preventing part is formed to have a rounded tip.

5. The cell culture assay of claim 1 , wherein the leak-preventing part is formed to have a tip having a square cross section.

6. The cell culture assay of claim 1 , wherein the scaffold channel and the microfluidic channel are disposed in an alternate and repetitive manner.

7. The cell culture assay of claim 1 , wherein the microfluidic channel has both ends extending in a direction being distant from the scaffold channel.

8. The cell culture assay of claim 1 , wherein the scaffold is injected in a fluid form to gelate or is formed of curable materials.

9. The cell culture assay of claim 1 , wherein the scaffold is formed of one of alginate, collagen, peptide, fibrin, hyaluronic acid, agarose, polyhydroxyethylmethacrylate (PHEMA), polyvinyl alcohol (PVA), Poly(ethylene glycol)(PEG), Poly(ethylene oxide)(PEO), polyethylene (glycol) diacrylate (PEGDA), gelatin, matrigel, poly(L-lactic acid)(PLLA), carboxymethylcellulose, SAP, PHEMA-MMA, dextran, and chitosan or a mixture of two or more materials.

Assignees

• Univ Korea Res & Bus Found

Inventors

• Chung Seok
• Han Se Woon
• Shin Yoo Jin
• Byun Jae Young

Classifications

• C12M23/34

  Internal compartments or partitions · CPC title

• G01N33/5005 Primary

  involving human or animal cells (immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025) · CPC title

• C12M41/36

  of biomass, e.g. colony counters or by turbidity measurements (electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404) · CPC title

• B01L3/502707

  characterised by the manufacture of the container or its components · CPC title

• C12M23/16 Primary

  Microfluidic devices; Capillary tubes (integrated microfluidic structures B01L3/5027; microreactors B01J19/0093) · CPC title