Analogs of temporin-SHa and uses thereof

Patent metadata

Field	Value
Publication number	US-9522942-B2
Application number	US-201013257018-A
Country	US
Kind code	B2
Filing date	Mar 18, 2010
Priority date	Mar 19, 2009
Publication date	Dec 20, 2016
Grant date	Dec 20, 2016

Abstract

The present invention relates to novel antimicrobial peptides, to pharmaceutical compositions comprising said peptides, and to the uses thereof, in particular as antimicrobial drugs, disinfectants, pesticides or preservatives. The present invention also relates to a transgenic plant expressing said novel peptides.

First claim

The invention claimed is:

1. A non-natural peptide of between 13 and 100 amino acids selected from the group consisting of:
a) a peptide containing the sequence F-L-X 1 -G-I-V-G-M-L-G-K-L-F (SEQ ID NO: 2) or a pharmaceutically acceptable salt thereof;
b) a retropeptide or retro-inverso peptide of a peptide containing the sequence F-L-X 1 -G-I-V-G-M-L-G-K-L-F (SEQ ID NO: 2) or a pharmaceutically acceptable salt thereof; and
c) a peptide containing the sequence of SEQ ID NO: 2 shortened by 1 or 2 amino acids at the N-terminal end or a pharmaceutically acceptable salt thereof;
wherein said non-natural peptide or said pharmaceutically acceptable salt thereof as shown in group a), b) and c) exhibits antimicrobial activity and wherein X 1 is an amino acid selected from the group consisting of R (arginine), H (histidine) and K (lysine).

2. The non-natural peptide according to claim 1 , wherein X 1 represents K (lysine).

3. A method for treating a microbial infection comprising administering a therapeutically effective dose of a non-natural peptide according to claim 1 to a subject in need of treatment for said microbial infection.

4. The method according to claim 3 , wherein the microbial infection is an infection due to a bacterium, a virus, a fungus or a parasite.

5. The method according to claim 4 , wherein the parasite belongs to the genus Leishmania.

6. A method for treating an infection of a plant by a phytopathogen comprising administering an effective dose of a non-natural peptide according to claim 1 to said plant.

7. A method for improving conservation of a food product comprising applying an effective dose of a non-natural peptide according to claim 1 to said food product.

8. A method of disinfecting a surface, a liquid or a gas comprising applying an effective dose of a non-natural peptide according to claim 1 to said surface, liquid or gas.

9. The non-natural peptide according to claim 1 , wherein X 1 represents H (histidine).

10. The non-natural peptide according to claim 1 , wherein X 1 represents R (arginine).

11. The non-natural peptide according to claim 1 , wherein said non-natural peptide has a size of 13 amino acids.

12. The non-natural peptide according to claim 1 , wherein said non-natural peptide contains the amino acid sequence of SEQ ID NO: 2.

13. The non-natural peptide according to claim 1 , wherein said non-natural peptide is a retropeptide or retro-inverso peptide of a peptide containing the amino acid sequence of SEQ ID NO: 2.

14. The non-natural peptide according to claim 1 , wherein said non-natural peptide contains the amino acid sequence of SEQ ID NO: 2 shortened by 1 or 2 amino acids at the N-terminal end.

15. The non-natural peptide according to claim 1 , wherein said pharmaceutically acceptable salt is a hydrochloric acid salt, hydrobromic acid salt, sulphuric acid salt, phosphoric acid salt; acetic acid salt, citric acid salt, maleic acid salt, malic acid salt, succinic acid salt, ascorbic acid salt, tartaric acid salt, sodium salt, potassium salt, calcium salt, magnesium salt or ammonium salt.

Assignees

• Ladram Ali
• Sereno Denis
• Abbassi Feten
• Oury Bruno
• Amiche Mohamed
• Nicolas Pierre
• Univ Pierre Et Marie Curie Paris 6
• Inst De Rech Pour Le Dev
• Centre Nat Rech Scient

Inventors

• Ladram Ali
• Sereno Denis
• Abbassi Feten
• Oury Bruno
• Amiche Mohamed
• Nicolas Pierre

Classifications

• A61P33/10

  Anthelmintics · CPC title

• A61P31/10

  Antimycotics · CPC title

• A61P31/04

  Antibacterial agents · CPC title

• A61P31/12

  Antivirals · CPC title

• C12N15/8282

  for fungal resistance · CPC title