Method and a system for producing thermolabile nanoparticles with controlled properties and nanoparticles matrices made thereby

Patent metadata

Field	Value
Publication number	US-9522378-B2
Application number	US-201514644463-A
Country	US
Kind code	B2
Filing date	Mar 11, 2015
Priority date	Aug 4, 2011
Publication date	Dec 20, 2016
Grant date	Dec 20, 2016

Abstract

This disclosure relates to a method and a system of producing nanoparticles and nanoparticle matrices of thermolabile, biocompatible matrix materials, like lipids and biopolymers with controlled properties. A prototype pulse-heat aerosol system is described for single-step production of free, thermolabile nanoparticles with sufficient control over size, morphology and crystallinity with controlled-release properties, for possible therapeutic, cosmetic or diagnostic use. Nanoparticles of the range 50 to 500 nm are obtained and are found suitable for controlled drugs delivery.

First claim

The invention claimed is:

1. A system for production of thermolabile nanoparticles from thermolabile biocompatible matrix material(s) selected from the group consisting of lipids, biopolymers and proteins, optionally with therapeutic, cosmetic or protein active agents, comprising:
an atomizer for atomizing a solution of the thermolabile, biocompatible matrix material(s) in aqueous or organic solvent(s), with the active agents, by mixing the solution with carrier gas to create droplets from the solution;
a pulse-heating zone for pulse heating the droplets suspended in the carrier gas to form a heated aerosol comprising the suspended nanoparticles and carrier gas under predetermined heat pulse of controlled magnitude and duration comprising exposing the suspended droplets to heating at a temperature in the range of 25° C. to 110° C. for a pulse duration between 0.1 to 1 seconds, to achieve control over nanoparticle properties;
a quenching zone for cooling the aerosol containing the suspended nanoparticles by dilution with a cooling gas, the quenching zone having means for supplying the cooling gas in a regulated manner; and
a sampling zone for collecting the nanoparticles produced.

2. The system as claimed in claim 1 , configured and adapted for passing laminar flow of the aerosol from the pulse-heat zone, introduced into the pulse-heat zone by the atomizer, through the quenching zone and to the sampling zone.

3. The system as claimed in claim 1 , wherein the pulse-heating zone is connected to the quenching zone and the sampling zone.

4. The system as claimed in claim 1 , wherein the pulse-heating zone and quenching zone further comprises a temperature controller.

5. The system as claimed in claim 1 , wherein the system is a modular system.

6. The system as claimed in claim 1 , wherein the atomizer is selected from the group consisting of collision air jet atomizer, atomization device based on ultrasonic, electrospray, evaporation-condensation, and filter-expansion-aerosol-generation (FEAG) principle of aerosol generation.

7. The system as claimed in claim 1 , further comprising a measurement device for measuring the mobility diameter of the nanoparticles, the measurement device selected from the group consisting of scanning mobility particle sizer (SMPS), electrical low pressure impactor (ELPI), and hypersonic impactor.

8. The system as claimed in claim 1 , wherein the pulse-heating zone has an internal diameter of 38 mm and a heated length of 80 mm.

9. The system as claimed in claim 1 , wherein the quenching zone comprises a perforated-wall diluter having holes each drilled at regular intervals up to a length of 100 mm.

10. The system as claimed in claim 1 , wherein the sampling zone comprises three outlets, one outlet for collecting the nanoparticles, one outlet for isoaxial sampling of the nanoparticles for measurements, and one outlet for exhaust.

11. The system as claimed in claim 1 , wherein the nanoparticles are collected using a particle collection system selected from the group consisting of an electrostatic precipitator (dry/wet), a cyclone, a filter, a settling chamber, and an impactor.

12. The system as claimed in claim 1 , wherein the carrier gas and cooling gas is selected from the group consisting of nitrogen gas and inert gas.

13. The system as claimed in claim 1 , wherein the organic solvent is selected from the group consisting of hydrocarbon, halogenated hydrocarbon, alcohol, ketone, and ester.

14. The system as claimed in claim 1 , wherein the organic solvents are selected from the group consisting of cyclohexane, chloroform, and dichloromethane.

15. The system as claimed in claim 1 , wherein the nanoparticles produced are of controlled size, morphology or structure, crystallinity and controlled-release properties.

16. The system as claimed in claim 1 , wherein the pulse-heating zone is connected to the quenching zone and the sampling zone to be configured for passing the aerosol from the pulse heating zone along with a gas stream line through the quenching zone.

17. The system of claim 1 , comprising the atomizer, the pulse-heating zone connected to a perforated dilutor, and the sampling zone comprising an isoaxial sampler.

18. The system as claimed in claim 1 , wherein the heat pulse is provided for heating the droplets suspended in carrier gas.

Assignees

• Indian Inst Technology Bombay

Inventors

• Venkataraman Chandra
• Pawar Amol Ashok

Classifications

• B01J13/04 Primary

  by physical processes, e.g. drying, spraying · CPC title

• B82Y5/00

  Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery · CPC title

• A61K8/0283

  Matrix particles · CPC title

• A61K8/361

  Carboxylic acids having more than seven carbon atoms in an unbroken chain; Salts or anhydrides thereof · CPC title

• A61K2800/56

  Compounds, absorbed onto or entrapped into a solid carrier, e.g. encapsulated perfumes, inclusion compounds, sustained release forms · CPC title