Method for assaying plasminogen in a liquid medium, associated compositions and kit

Patent metadata

Field	Value
Publication number	US-9518286-B2
Application number	US-201414198072-A
Country	US
Kind code	B2
Filing date	Mar 5, 2014
Priority date	Sep 7, 2011
Publication date	Dec 13, 2016
Grant date	Dec 13, 2016

Abstract

The invention relates to a method for assaying plasminogen in a sample comprising a step consisting in particular of reacting a streptokinase (R1), and a streptokinase activator, with a control solution or a diluted plasma sample, in which the streptokinase activator is selected from the group comprising a fibrin DD fragment and/or at least one DD fragment derivative.
The invention also relates to a liquid composition, a plasminogen assay kit for implementing this method and the use of a streptokinase activator selected from the group comprising a fibrin DD fragment and/or at least one DD fragment derivative.

First claim

The invention claimed is:

1. A method for assaying plasminogen in a sample comprising steps consisting of:
a) storing a composition comprising a streptokinase and a streptokinase activator in a ready-to-use solution;
b) reacting the composition with a control solution or a diluted plasma sample;
c) tracking said reaction;
d) determining the amount of plasminogen in the control solution or in the diluted plasma sample, as a function of the result of the tracking of said reaction, wherein the streptokinase activator is a fibrin DD fragment, at least one DD fragment derivative, or both a fibrin DD fragment and at least one DD fragment derivative.

2. The method according to claim 1 , wherein the reaction step b) is performed in the presence of an anticoagulant.

3. The method according to claim 1 , wherein the storage step is performed at a temperature of between −20° C. and 37° C.

4. The method according to claim 1 , wherein the storage step is performed for a period greater than 12 hours.

5. The method according to claim 1 , further comprising before the reaction step b), a step of pre-incubating the composition comprising the streptokinase and the streptokinase activator, at around 37° C.

6. The method according to claim 1 , wherein the reaction step b) is performed at around 37° C.

7. The method according to claim 1 , wherein the reaction step b) is stopped by an acidification step.

8. A method for assaying plasminogen in a sample comprising:
storing a composition comprising streptokinase and a streptokinase activator in a ready-to-use solution;
reacting the composition with a control solution or a diluted plasma sample;
tracking the reaction of the streptokinase and the streptokinase activator with the control solution of the diluted plasma sample; and
determining an amount of plasminogen in the control solution or in the diluted plasma sample based on the result of the tracking of the reaction,
wherein the streptokinase activator is at least one selected from the group consisting of a fibrin DD fragment and at least one DD fragment derivative.

9. The method according to claim 8 , wherein the tracking step is performed by using a chromogenic substrate specific for plasmin and plasmin-streptokinase complexes.

10. The method according to claim 8 , wherein the reaction step is performed in the presence of an anticoagulant.

11. The method according to claim 8 , wherein the storage step is performed at a temperature of between −20° C. and 37° C.

12. The method according to claim 8 , wherein the storage step is performed for a period greater than 12 hours.

Assignees

• Sysmex Corp

Inventors

• Amiral Jean

Classifications

• G01N33/86

  involving blood coagulating time {or factors, or their receptors} · CPC title

• C12Q1/56 Primary

  involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen · CPC title