Bacterial strain Rhodococcus aetherivorans VKM Ac-2610D producing nitrile hydratase, method of its cultivation and method for producing acrylamide

Patent metadata

Field	Value
Publication number	US-9518279-B2
Application number	US-201314140150-A
Country	US
Kind code	B2
Filing date	Dec 24, 2013
Priority date	Dec 27, 2012
Publication date	Dec 13, 2016
Grant date	Dec 13, 2016

Abstract

The invention relates to a bacterial strain belonging to the genus Rhodococcus which is a producer of a nitrile hydratase. The invention also relates to a method for producing acrylamide by hydration of acrylonitrile using a biomass of the bacterial strain and to a method of culturing the bacterial strain.

First claim

The invention claimed is:

1. A method for producing acrylamide, the method comprising hydration of acrylonitrile using a biomass of a bacterial strain belonging to the genus Rhodococcus and having a nitrile hydratase activity, wherein hydration is carried out at a working concentration of acrylonitrile which is no more than 0.5%, and the bacterial strain is Rhodococcus aetherivorans VKM Ac-2610D.

2. The method of claim 1 , wherein acrylonitrile is mixed in an aqueous suspension of the biomass of the bacterial strain to form a reaction solution; and hydration is carried out so that the working concentration of acrylonitrile in the reaction solution is maintained at no more than 0.5%.

3. The method of claim 1 , wherein hydration is followed by isolation of acrylamide.

4. The method of claim 1 , wherein the biomass of the bacterial strain is at about 100-1000 g, or 200-1000 g, or 200-800 g, or 300-600 g, or about 400-500 g, or less than 1000 g, or less than 500 g, or less than 400 g on a dry weight of strain per 1 ton of a final product.

5. The method of claim 4 , wherein the final product contains acrylamide at a concentration of at least 40%; or at least 41%; or at least 42%; or at least 43%; or at least 44%; or 40-55%; or 41-55%; 42-55%; or 43-55%; or 44-55%; or at least 45%; or at least 46%; or at least 47%; or 45-55%; or 45-54%; or 45-53%; or 45-52%; or 45-51%; or 45-50%; or 45-49%; or 46-55%; or 46-54%; or 46-53%; or 46-52%; or 46-51%; or 46-50%; or 46-49%; or 47-55%; or 47-54%; or 47-53%; or 47-52%; or 47-51%; or 47-50%; or 47-49%.

6. The method of claim 1 , wherein hydration of acrylonitrile is carried out at a temperature of 10-23° C.

7. The method of claim 1 , wherein hydration of acrylonitrile is carried out at a pH of 6.8-8.4.

8. The method of claim 1 , wherein the biomass of the bacterial strain is at about 100-1000 g on a dry weight of strain per 1 ton of a final product.

9. The method of claim 1 , wherein the biomass of the bacterial strain is at about 200-1000 g on a dry weight of strain per 1 ton of a final product.

10. The method of claim 1 , wherein the biomass of the bacterial strain is at about 200-800 g on a dry weight of strain per 1 ton of a final product.

11. The method of claim 1 , wherein the biomass of the bacterial strain is at about 300-600 g on a dry weight of strain per 1 ton of a final product.

12. The method of claim 1 , wherein the biomass of the bacterial strain is at about 400-500 g on a dry weight of strain per 1 ton of a final product.

13. The method of claim 1 , wherein the biomass of the bacterial strain is less than 1000 g on a dry weight of strain per 1 ton of a final product.

14. The method of claim 1 , wherein the biomass of the bacterial strain is less than 500 g on a dry weight of strain per 1 ton of a final product.

15. The method of claim 1 , wherein the biomass of the bacterial strain is less than 400 g on a dry weight of strain per 1 ton of a final product.

16. The method of claim 4 , wherein the final product contains acrylamide at a concentration of 47-55%.

17. The method of claim 4 , wherein the final product contains acrylamide at a concentration of 47-54%.

18. The method of claim 4 , wherein the final product contains acrylamide at a concentration of 47-52%.

19. The method of claim 4 , wherein the final product contains acrylamide at a concentration of 47-52%.

20. The method of claim 4 , wherein the final product contains acrylamide at a concentration of 47-49%.

Assignees

• Kemira Oyj

Inventors

• Kozulin Sergey Vladimirovich
• Kozulina Tatiana Nicolaevna
• Kozulin Alexey Sergeevich

Classifications

• C12P13/02 Primary

  Amides, e.g. chloramphenicol {or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes (peptides C12P21/00 or C07K)} · CPC title

• C12Y402/01084

  Nitrile hydratase (4.2.1.84) · CPC title

• C12N1/20 Primary

  Bacteria; Culture media therefor · CPC title

• C12R1/00

  Chemistry & Metallurgy · mapped topic

• C12R2001/00

  Microorganisms {; Processes using microorganisms} · CPC title