Droplet manipulation using gas-phase standing-wave ultrasound fields in MS sources

Patent metadata

Field	Value
Publication number	US-9514924-B2
Application number	US-201514719665-A
Country	US
Kind code	B2
Filing date	May 22, 2015
Priority date	Nov 22, 2011
Publication date	Dec 6, 2016
Grant date	Dec 6, 2016

Abstract

An ion source for a mass spectrometer is disclosed comprising an ionization device which emits a stream of droplets and one or more ultrasonic transmitters which create one or more acoustic standing waves. The acoustic standing waves may be used to further nebulize the stream of droplets and induce internal mixing of the droplets.

First claim

The invention claimed is:

1. Apparatus for a mass spectrometer comprising:
a device arranged and adapted to emit a stream of droplets;
one or more ultrasonic transmitters or sonotrodes arranged and adapted to create one or more acoustic standing waves downstream of said device;
wherein said one or more acoustic standing waves are arranged and adapted:
to induce internal mixing of said stream of droplets;
to further nebulise said stream of droplets; and
to result in a reduction of an average size of droplets in said stream of droplets.

2. Apparatus as claimed in claim 1 , wherein said droplets predominantly comprise charged droplets.

3. Apparatus as claimed in claim 1 , wherein said droplets predominantly comprise uncharged or neutral droplets.

4. Apparatus as claimed in claim 1 , wherein said device comprises an Atmospheric Pressure Ionisation (“API”) ionisation device.

5. Apparatus as claimed in claim 1 , wherein said one or more ultrasonic transmitters or sonotrodes comprises an ultrasonic transmitter or sonotrode and a reflector.

6. Apparatus as claimed in claim 1 , wherein one or more ultrasonic transmitters or sonotrodes comprises two ultrasonic transmitters or sonotrodes.

7. Apparatus as claimed in claim 1 , wherein said one or more ultrasonic transmitters are arranged and adapted to emit ultrasonic waves having a frequency in the range: (i) 20-30 kHz; (ii) 30-40 kHz; (iii) 40-50 kHz; (iv) 50-60 kHz; (v) 60-70 kHz; (vi) 70-80 kHz; (vii) 80-90 kHz; (viii) 90-100 kHz; and (ix) >100 kHz.

8. Apparatus as claimed in claim 1 , wherein said one or more ultrasonic transmitters are positioned so that said one or more acoustic standing waves interact with said stream of droplets.

9. Apparatus as claimed in claim 1 , wherein said one or more acoustic standing waves are arranged and adapted to move or translate said stream of droplets.

10. Apparatus as claimed in claim 1 , wherein in a mode of operation said one or more acoustic standing waves are arranged so as to trap said droplets for a period of time.

11. Apparatus as claimed in claim 1 , further comprising a device arranged and adapted to introduce or mix a reagent or reagent ions with said droplets.

12. Apparatus as claimed in claim 11 , wherein said reagent or reagent ions react or interact with said droplets.

13. Apparatus as claimed in claim 11 , wherein said reagent or reagent ions react or interact via Electron Transfer Dissociation, Electron Capture Dissociation, ozonolysis, Hydrogen-Deuterium exchange (“HDx”), charge reduction, photo dissociation or thermal dissociation.

14. Apparatus as claimed in claim 1 , further comprising a control system arranged and adapted to control a residence time or interaction time between droplets and a reagent or reagent ions.

15. Apparatus as claimed in claim 1 , wherein said one or more ultrasonic transmitters are arranged and adapted to create one or more gas phase acoustic standing waves.

16. Apparatus as claimed in claim 1 , further comprising one or more grid electrodes for applying an electric field to droplets held in said one or more acoustic standing waves.

17. Apparatus as claimed in claim 16 , wherein said one or more grid electrodes are at least partially acoustically transparent at a frequency at which said one or more ultrasonic transmitters emit ultrasonic waves.

18. A mass spectrometer comprising
a device arranged and adapted to emit a stream of droplets;
one or more ultrasonic transmitters or sonotrodes arranged and adapted to create one or more acoustic standing waves downstream of said device;
wherein said one or more acoustic standing waves are arranged and adapted:
to induce internal mixing of said stream of droplets;
to further nebulise said stream of droplets; and
to result in a reduction of an average size of droplets in said stream of droplets.

19. A mass spectrometer as claimed in claim 18 , wherein said mass spectrometer comprises an inlet.

20. A mass spectrometer as claimed in claim 19 , wherein said inlet leads from a substantially atmospheric pressure region to a substantially sub-atmospheric pressure region.

21. A mass spectrometer as claimed in claim 20 , wherein analyte molecules or ions are arranged to emerge from said one or more acoustic standing waves adjacent said inlet so that said analyte molecules or ions enter said mass spectrometer via said inlet.

22. A mass spectrometer as claimed in claim 21 , further comprising a gas phase ion mobility spectrometer or separator, wherein said ion mobility spectrometer or separator is arranged and adapted to separate analyte ions temporally according to their ion mobility.

23. A method of mass spectrometry comprising:
emitting a stream of droplets; and
causing said stream of droplets to interact with one or more acoustic standing waves;
whereby said one or more acoustic standing waves are arranged and adapted:
to induce internal mixing of said stream of droplets;
to further nebulise said stream of droplets; and
to result in a reduction of the average size of droplets in said stream of droplets.

Assignees

• Micromass Ltd

Inventors

• Morris Michael Raymond
• Pringle Steven Derek
• Richardson Keith

Classifications

• H01J49/26

  Mass spectrometers or separator tubes · CPC title

• Y10T137/0391

  Affecting flow by the addition of material or energy · CPC title

• H01J49/24

  Vacuum systems, e.g. maintaining desired pressures · CPC title

• Y10T137/206

  Flow affected by fluid contact, energy field or coanda effect [e.g., pure fluid device or system] · CPC title

• H01J49/0454

  with means for vaporising using mechanical energy, e.g. by ultrasonic vibrations · CPC title