Telecentric lens assembly providing a collimated light space

Patent metadata

Field	Value
Publication number	US-9513470-B1
Application number	US-201414173290-A
Country	US
Kind code	B1
Filing date	Feb 5, 2014
Priority date	Feb 5, 2013
Publication date	Dec 6, 2016
Grant date	Dec 6, 2016

Abstract

Systems and methods for imaging a target are provided. A system includes a detector and a substantially bitelecentric lens assembly positioned between the detector and the target. A first optical assembly is configured to focus light received from the substantially bitelecentric lens assembly onto the detector. The substantially bitelecentric lens assembly and the first optical assembly are configured to produce a collimated light space between the substantially bitelecentric lens assembly and the first optical assembly. A second optical assembly is positioned within the collimated light space.

First claim

What is claimed is:

1. An imaging system comprising:
a detector;
a substantially bitelecentric lens assembly, positioned between the detector and a target;
a first optical assembly configured to focus light received from the substantially bitelecentric lens assembly onto the detector, the substantially bitelecentric lens assembly and the first optical assembly being configured to produce a collimated light space between the substantially bitelecentric lens assembly and the first optical assembly; and
a second optical assembly positioned within the collimated light space.

2. The imaging system of claim 1 , wherein the second optical assembly is configured to be modular, such that the second optical assembly can be removed and replaced with a third optical assembly without disturbance of the detector, the substantially bitelecentric lens assembly, and the first optical assembly.

3. The imaging system of claim 2 , wherein each of the second optical assembly and the third optical assembly are filters in a set of excitation and emission filters selected to support fluorescent probes, the set including at least one filter configured to selectively passing light from blue fluorescent protein, at least one filter configured to selectively passing light from red fluorescent protein, and at least one filter configured to selectively passing light from one of green calcium and thallium-sensitive fluorescent dye.

4. The imaging system of claim 1 , wherein each of the second optical assembly and the third optical assembly comprise at least one filter configured in a filter changer.

5. The imaging system of claim 1 , wherein the first optical assembly is a substantially object-space telecentric lens assembly.

6. The imaging system of claim 1 , wherein the target is an integrated circuit and the imaging system is part of a integrated circuit testing system.

7. The imaging system of claim 1 , wherein the second optical assembly is a beam splitter.

8. The imaging system of claim 1 , wherein the second optical assembly is a dichroic mirror.

9. The imaging system of claim 1 , wherein the second optical assembly is at least one of a microfilter array, a microlens array, a micropolarizer array, a micro-prism array, and a micro-diffraction grating array.

10. The imaging system of claim 1 , wherein the target is a microplate, and the imaging system is part of a plate reader.

11. The imaging system of claim 1 , wherein a lens arrangement comprising the substantially bitelecentric lens assembly and the first optical assembly has a focal ratio less than F/2.

12. The imaging system of claim 1 , wherein the second optical assembly is one of a longpass filter, a shortpass filter, a neutral density filter, a polarizer, and a dichroic filter.

13. A method for imaging a target comprising:
aligning a target in a field of view of an object-side telecentric lens assembly having a first microfilter array in a collimated light space;
imaging the target using the first microfilter array with an imaging system associated with the telecentric lens;
exchanging a second microfilter array for the first microfilter array without disturbing the alignment of the target at the telecentric lens; and
imaging the target using the second microfilter array with the image system.

14. The method of claim 13 , wherein the object-side telecentric lens assembly has a focal ratio less than F/2.

15. A kinetic imaging plate reader system comprising:
a detector;
a lens assembly, positioned between the detector and a microplate;
an optical assembly configured to focus light received from the lens assembly onto the detector, the lens assembly and the optical assembly being configured to produce a collimated light space between the lens assembly and the optical assembly; and
a first filter positioned within the collimated light space, the first filter being configured to be modular, such that the first filter can be removed and replaced with a second filter without substantial disturbance of any of the detector, the lens assembly, and the optical assembly.

16. The kinetic imaging plate reader system of claim 15 , further comprising an automated liquid handling system configured to dispense liquid into a plurality of wells in the microplate.

17. The kinetic imaging plate reader system of claim 16 , wherein the microplate is positioned above each of the detector, the lens assembly, and the optical assembly such that the microplate is freely accessible to the automated liquid handling system.

18. The kinetic imaging plate reader system of claim 15 , wherein the first and second filters are part of a set of excitation and emission filters selected to support fluorescent probes, the set of filters including at least one filter configured to selectively passing light from blue fluorescent protein, at least one filter configured to selectively passing light from red fluorescent protein, and at least one filter configured to selectively passing light from one of green calcium and thallium-sensitive fluorescent dye.

Assignees

• Univ Vanderbilt

Inventors

• Weaver Charles David

Classifications

• G02B13/22 Primary

  Telecentric objectives or lens systems · CPC title

• G01N21/253

  for batch operation, i.e. multisample apparatus (analytical automats G01N35/00) · CPC title

• G01N21/6452

  Individual samples arranged in a regular 2D-array, e.g. multiwell plates · CPC title

• G02B27/141

  using dichroic mirrors · CPC title

• B01L3/5085

  for multiple samples, e.g. microtitration plates · CPC title