Devices and methods for manipulating components in a fluid sample

Patent metadata

Field	Value
Publication number	US-9513205-B2
Application number	US-201514590659-A
Country	US
Kind code	B2
Filing date	Jan 6, 2015
Priority date	Oct 26, 2012
Publication date	Dec 6, 2016
Grant date	Dec 6, 2016

Abstract

Devices for sorting components (e.g., cells) contained in a liquid sample are provided. In certain aspects, the devices include a magnetic separation device and an acoustic concentrator device fluidically coupled to magnetic separation device. Aspects of the invention further include methods for sorting cells in a liquid sample, and systems, and kits for practicing the subject methods.

First claim

What is claimed is:

1. A fluidic device for manipulating components in a liquid sample, the device comprising:
a) a magnetic separator;
b) an acoustic concentrator fluidically coupled to the magnetic separator; and
c) a fluid flow channel between the magnetic separator and the acoustic concentrator, wherein the magnetic separator generates a magnetic field that is spatially separated from the acoustic field generated by the acoustic concentrator.

2. The device according to claim 1 , wherein the acoustic concentrator is fluidically coupled to an output of the magnetic separator.

3. The device according to claim 1 , wherein the liquid sample has a viscosity of about 0.2 cP to about 1.5 cP.

4. The device according to claim 1 , wherein the liquid sample comprises cells.

5. The device according to claim 1 , wherein the device has an output flow rate of about 30 to 100 μL/min.

6. The device according to claim 1 , wherein the magnetic separator comprises a permanent magnet.

7. The device according to claim 1 , wherein the magnetic separator comprises a magnetic field guide.

8. The device according to claim 1 , wherein the acoustic concentrator comprises a flow channel comprising an inlet and an outlet, the inlet and outlet defining a fluid flow path therebetween; and
a vibration generator positioned adjacent to the fluid flow path and configured to produce an acoustic field in the fluid flow path.

9. The device according to claim 8 , wherein the vibration generator is a piezoelectric transducer.

10. The device according to claim 9 , wherein the vibration generator comprises lead zirconate titanate.

11. The device according to claim 1 , comprising a processor configured to apply a magnetic field using the magnetic separator.

12. The device according to claim 1 , comprising a processor configured to control the acoustic concentrator.

13. A flow cytometric system comprising:
i) a flow cytometric sample fluidic subsystem, the flow cytometric sample fluidic subsystem comprising: a) a magnetic separator; b) an acoustic concentrator fluidically coupled to the magnetic separator; and c) a fluid flow channel between the magnetic separator and the acoustic concentrator, wherein the magnetic separator generates a magnetic field that is spatially separated from the acoustic field generated by the acoustic concentrator; and ii) a flow cytometer fluidically coupled to the output of the flow cytometric sample fluidic subsystem.

Assignees

• Becton Dickinson Co

Inventors

• Yu Liping
• Warner Brian David
• Trotter Joseph T

Classifications

• G01N2001/4038

  electric methods, e.g. electromigration, electrophoresis, ionisation · CPC title

• B01L3/502761

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title

• B01L2400/0439

  ultrasonic vibrations, vibrating piezo elements · CPC title

• G01N15/14

  Optical investigation techniques, e.g. flow cytometry · CPC title

• G01N1/34

  Purifying; Cleaning {(processes or apparatus for extracting or separating nucleic acids from biological samples C12N15/1003)} · CPC title