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US-2024230643-A9 · Jul 11, 2024 · US
Use of N-acetylneuraminic acid aldolase in catalytic synthesis of N-acetylneuraminic acid
US9512420B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9512420-B2 |
| Application number | US-201314396317-A |
| Country | US |
| Kind code | B2 |
| Filing date | Nov 27, 2013 |
| Priority date | Oct 16, 2013 |
| Publication date | Dec 6, 2016 |
| Grant date | Dec 6, 2016 |
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Abstract
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It discloses a use of N-acetylneuraminic acid aldolase with an amino acid sequence as shown in SEQ ID NO: 2 in catalytic synthesis of N-acetylneuraminic acid. The preparation of N-acetylneuraminic acid is to use the N-acetylneuraminic acid aldolase with the amino acid sequence as shown in SEQ ID NO: 2 as a catalyst, and N-acetylmannosamine and pyruvic acid as substrates.
First claim
Opening claim text (preview).
What is claimed is: 1. A method for using Corynebacterium N-acetylneuraminic acid aldolase for preparing N-acetylneuraminic acid with N-acetylmannosamine and pyruvic acid in a buffer. 2. The method according to claim 1 , wherein the concentration of N-acetylneuraminic acid aldolase is from 0.36 to 300 U·mL −1 , the concentration of N-acetylmannosamine is from 100 to 1000 mmol·L −1 and the concentration of pyruvic acid is from 100 to 2000 mmol·L −1 ; a reaction temperature is between 25 and 60° C.; and a reaction time is between 0.1 and 12 hours. 3. The method according to claim 1 , wherein the buffer is a Tris-HCl buffer at pH between 7 and 8.8, or a glycine-NaOH buffer at pH between 9.0 and 9.5. 4. The method according to claim 1 , wherein the N-acetylneuraminic acid aldolase is produced by E. coli that is transformed by an expression vector that contains N-acetylneuraminic acid aldolase cDNA having the nucleic acid sequence as shown in SEQ ID NO: 1. 5. The method according to claim 4 , the expression vector is a pET-28a vector.
Assignees
Inventors
Classifications
N-Acetylneuraminate lyase (4.1.3.3) · CPC title
using catalysts, e.g. selective catalysts · CPC title
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression · CPC title
- C12N9/88Primary
Lyases (4.) · CPC title
Preparation of nitrogen-containing carbohydrates · CPC title
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- What does patent US9512420B2 cover?
- It discloses a use of N-acetylneuraminic acid aldolase with an amino acid sequence as shown in SEQ ID NO: 2 in catalytic synthesis of N-acetylneuraminic acid. The preparation of N-acetylneuraminic acid is to use the N-acetylneuraminic acid aldolase with the amino acid sequence as shown in SEQ ID NO: 2 as a catalyst, and N-acetylmannosamine and pyruvic acid as substrates.
- Who is the assignee on this patent?
- Nanjing Univ Of Tech, Nanjing University Of Technology
- What technology area does this patent fall under?
- Primary CPC classification C12N9/88. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Dec 06 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).