Methods for producing an immune response to tuberculosis

Patent metadata

Field	Value
Publication number	US-9506922-B2
Application number	US-201514590810-A
Country	US
Kind code	B2
Filing date	Jan 6, 2015
Priority date	Nov 20, 2009
Publication date	Nov 29, 2016
Grant date	Nov 29, 2016

Abstract

Methods for producing an immune response to Mycobacterium tuberculosis (Mtb) are disclosed herein. In several examples, the immune response is a protective immune response. In additional embodiments, methods are disclosed for inhibiting an infection with Mtb, preventing an infection with Mtb, or treating an infection with Mtb. Pharmaceutical compositions for the inhibition, prevention and/or treatment of tuberculosis are also disclosed.

First claim

We claim:

1. A method for producing an immune response in a subject, comprising administering to the subject a therapeutically effective amount of a viral expression vector comprising a promoter operably linked to a polynucleotide encoding a polypeptide,
wherein the polypeptide
(a) comprises the amino acid sequence set forth as SEQ ID NO: 2; or
(b) consists of contiguous amino acids 141-149 of the amino acid sequence set forth as SEQ ID NO: 2, thereby inducing an immune response to the polypeptide.

2. The method of claim 1 , wherein the polypeptide consists of amino acids 141-149 of the amino acid sequence set forth as SEQ ID NO: 2.

3. The method of claim 1 , further comprising administering a therapeutically effective amount of an adjuvant to the subject.

4. The method of claim 1 , wherein the subject is at risk for infection with Mycobacterium tuberculosis (Mtb).

5. The method of claim 1 , wherein the polypeptide consists of the amino acid sequence set forth as SEQ ID NO: 2.

6. The method of claim 1 , wherein the viral expression vector is a poxvirus vector.

7. The method of claim 6 , wherein the poxvirus vector is a vaccinia virus vector.

8. The method of claim 7 , wherein the vaccinia virus vector is a non-replicating vaccinia virus.

9. The method of claim 6 , wherein the poxvirus vector is a modified vaccinia Ankara virus.

10. The method of claim 6 , wherein the polynucleotide is operably linked to a poxviral promoter.

11. The method of claim 1 , wherein the subject is infected with Mycobacterium tuberculosis.

12. The method of claim 11 , wherein the subject has a latent infection with Mycobacterium tuberculosis.

13. A method for producing an immune response in a subject, comprising
administering to the subject a therapeutically effective amount of a viral expression vector comprising a polynucleotide encoding a polypeptide, wherein the polypeptide comprises the amino acid sequence set forth as SEQ ID NO: 2,
thereby inducing an immune response to the polypeptide.

14. The method of claim 13 , further comprising administering to the subject a therapeutically effective amount of an adjuvant.

15. The method of claim 13 , wherein the subject is at risk for infection with Mtb.

16. The method of claim 13 , wherein the viral expression vector is a poxvirus vector.

17. The method of claim 16 , wherein the poxvirus vector is a vaccinia virus vector.

18. The method of claim 17 , wherein the vaccinia virus vector is a non-replicating vaccinia virus.

19. The method of claim 16 , wherein the poxvirus vector is a modified vaccinia Ankara virus.

20. The method of claim 16 , wherein the polynucleotide is operably linked to a poxviral promoter.

21. The method of claim 13 , wherein the subject is infected with Mycobacterium tuberculosis.

22. The method of claim 21 , wherein the subject has a latent infection with Mycobacterium tuberculosis.

Assignees

• Univ Oregon Health & Science
• Us Veterans Affairs
• Us Gov Veterans Affairs

Inventors

• Lewinsohn David M
• Lewinsohn Deborah A

Classifications

• A61P31/06

  for tuberculosis · CPC title

• A61P37/04

  Immunostimulants · CPC title

• A61P31/16

  for influenza or rhinoviruses · CPC title

• G01N2469/10

  Detection of antigens from microorganism in sample from host · CPC title

• G01N33/5695 Primary

  Mycobacteria · CPC title