Method and system for selective isolation of target biological molecules in a general purpose system

Patent metadata

Field	Value
Publication number	US-9506847-B2
Application number	US-201414285099-A
Country	US
Kind code	B2
Filing date	May 22, 2014
Priority date	Aug 4, 2008
Publication date	Nov 29, 2016
Grant date	Nov 29, 2016

Abstract

Embodiments of the present techniques provide systems and methods for isolating particular classes of biological molecules, for example, proteins or nucleic acids, from mixtures of biological components. The methods use solutions that react with the biological molecules to enhance their adsorption by substrates, allowing contaminants to be washed away from the targeted molecules. Embodiments include automated systems that can be used to implement the technique with no or minimal intervention. Other embodiments include separation column technologies that may be used in the techniques.

First claim

What is claimed is:

1. An apparatus for isolating biological molecules from a sample, comprising:
a substrate having a surface configured to adsorb a plurality of types of biological molecules, wherein the plurality of types of biological molecules comprises at least nucleic acids and proteins;
a plurality of reservoirs, wherein each reservoir of the plurality of reservoirs houses a solution adapted to extract a specific type of biological molecule from the sample and to enhance adsorption of the specific type of biological molecule to the surface, and each solution housed in a respective reservoir of the plurality of reservoirs is adapted to extract and enhance adsorption of a different type of biological molecule from the other solutions housed in the other reservoirs;
a valve coupled to the plurality of reservoirs via tubing;
an injection port coupled to the valve and configured to receive the sample;
a reaction mixing tube coupled to the valve and configured to receive the sample and a selected solution from one reservoir of the plurality of reservoirs; and
a processor programmed to automatically select which of the solutions from the plurality of reservoirs should contact with the sample based at least in part on the type of biological molecule to be isolated, to control the valve to cause the selected solution and the sample to flow to the reaction mixing tube for mixing, and to control the valve to cause a mixture of the selected solution and the sample to be diverted from the reaction mixing tube to the substrate.

2. The apparatus of claim 1 , wherein the types of biological molecules further comprise polysaccharides and fragments thereof.

3. The apparatus of claim 1 , wherein the substrate is housed in a disposable, interchangeable cartridge.

4. The apparatus of claim 3 , wherein the cartridge comprises an identification component to enable the processor to identify the type of biological molecule to be isolated.

5. The apparatus of claim 4 , wherein the identification component comprises a bar code, RFID, machine-readable text, color coding, or other machine-readable encoding schemes and the processor comprises a reader configured to read the identification component.

6. The apparatus of claim 1 , further comprising a detector configured to generate a signal representing a property of an eluate from the substrate.

7. The apparatus of claim 6 , wherein the processor is further programmed to receive the signal, to analyze the signal, and to identify molecules present in the eluate based on the signal.

8. The apparatus of claim 6 , further comprising a sample collection device configured to collect the eluate.

9. An apparatus for isolating biological molecules from a sample, comprising:
a separation column configured to facilitate isolation of biological molecules from the sample, wherein the separation column comprises:
a substrate having a surface configured to adsorb a plurality of types of biological molecules, wherein the plurality of types of biological molecules comprises at least nucleic acids and proteins;

a plurality of reservoirs, wherein each reservoir of the plurality of reservoirs houses a solution adapted to extract a specific type of biological molecule from the sample and to enhance adsorption of the specific type of biological molecule to the surface, and each solution housed in a respective reservoir of the plurality of reservoirs is adapted to extract and enhance adsorption of a different type of biological molecule from the other solutions housed in the other reservoirs;
a valve coupled to the plurality of reservoirs via tubing;
an injection port coupled to the valve and configured to receive the sample;
a reaction mixing tube coupled to the valve and configured to receive the sample and a selected solution from one reservoir of the plurality of reservoirs; and
a processor programmed to automatically select which of the solutions from the plurality of reservoirs should contact with the sample based at least in part on the type of separation column to be utilized, to control the valve to cause the selected solution from the plurality of reservoirs and the sample to flow to the reaction mixing tube for mixing, and to control the valve to cause a mixture of the selected solution and the sample to be diverted from the reaction mixing tube to the separation column.

10. The apparatus of claim 9 , wherein the types of biological molecules further comprise polysaccharides and fragments thereof.

11. The apparatus of claim 9 , wherein the separation column comprises a disposable, interchangeable cartridge, and the substrate is housed in the cartridge.

12. The apparatus of claim 11 , wherein the processor is programmed to automatically select which of the solutions should contact with the sample based at least part on the type of biological molecule to be isolated.

13. The apparatus of claim 12 , wherein the cartridge comprises an identification component to enable the processor to identify the type of biological molecule to be isolated.

14. The apparatus of claim 13 , wherein the identification component comprises a bar code, RFID, machine-readable text, color coding, or other machine-readable encoding schemes and the processor comprises a reader configured to read the identification component.

15. The apparatus of claim 9 , further comprising a detector configured to generate a signal representing a property of an eluate from the substrate.

16. The apparatus of claim 15 , wherein the processor is further programmed to receive the signal, to analyze the signal, and to identify molecules present in the eluate based on the signal.

17. The apparatus of claim 9 , wherein the processor is further programmed to automatically determine electrophoresis conditions or sample collection conditions based at least in part on the type of separation column to be utilized.

Assignees

• Gen Electric

Inventors

• Duthie Robert Scott
• Tian Wei-Cheng
• Khurana Tarun

Classifications

• C12M23/16 Primary

  Microfluidic devices; Capillary tubes (integrated microfluidic structures B01L3/5027; microreactors B01J19/0093) · CPC title

• C12N15/1003

  Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor · CPC title

• G01N1/34 Primary

  Purifying; Cleaning {(processes or apparatus for extracting or separating nucleic acids from biological samples C12N15/1003)} · CPC title

• G01N27/447

  using electrophoresis · CPC title

• C12M47/12

  Purification (C12M47/04 takes precedence) · CPC title