Allele-specific amplification

Patent metadata

Field	Value
Publication number	US-9506111-B2
Application number	US-201314052416-A
Country	US
Kind code	B2
Filing date	Oct 11, 2013
Priority date	Oct 20, 2008
Publication date	Nov 29, 2016
Grant date	Nov 29, 2016

Abstract

The present invention includes a method of allele-specific amplification, utilizing an allele-specific oligonucleotide, at least partially complementary to more than one variant of the target sequence, but having a 3′-terminal nucleotide complementary to only one variant of the target sequence and having at least one nucleotide with a base covalently modified at the exocyclic amino group, wherein the allele-specific oligonucleotide is extended by a nucleotide-incorporating biocatalyst predominantly when hybridized to the variant of the target sequence for which it has said complementary 3′-terminal nucleotide.

First claim

The invention claimed is:

1. A kit for allele-specific amplification of a target sequence, which exists in the form of several variant sequences, comprising
(a) a first oligonucleotide, at least partially complementary to one or more variant of the target sequence; and
(b) a second oligonucleotide, at least partially complementary to one or more variant of the target sequence and having a 3′-terminal nucleotide complementary to only one variant of the target sequence; wherein said second oligonucleotide is selected from a group consisting of SEQ ID NOs: 4, 5, 7, 8, 10, 11, and 18.

2. An oligonucleotide for performing an allele-specific amplification of a target sequence, which exists in the form of several variant sequences, comprising
a sequence at least partially complementary to a portion of one or more variants of said target sequence;
a 3′-terminal nucleotide which is complementary to only one variant of said target sequence and
at least one nucleotide with a base covalently modified at the exocyclic amino group with a sequence selected from a group consisting of SEQ ID NOs: 4, 5, 7, 8, 10, 11 and 18.

Assignees

• Roche Molecular Systems Inc

Inventors

• Will Stephen G
• Tsan Alison
• Newton Nicolas

Classifications

• C12Q1/6858 Primary

  Allele-specific amplification · CPC title

• C12Q2535/125

  Allele specific primer extension · CPC title

• C12Q2525/117

  incorporating modified base · CPC title