Method for producing L-amino acid using microorganism having increased phosphate transporter activity

Patent metadata

Field	Value
Publication number	US-9506094-B2
Application number	US-201514796326-A
Country	US
Kind code	B2
Filing date	Jul 10, 2015
Priority date	May 13, 2013
Publication date	Nov 29, 2016
Grant date	Nov 29, 2016

Abstract

A method for producing an L-amino acid is provided. An L-amino acid is produced by culturing a coryneform bacterium having an L-amino acid-producing ability, which is modified so that the activity of a phosphate transporter is increased, in a medium, and collecting the L-amino acid from the medium.

First claim

The invention claimed is:

1. A method for producing an L-amino acid comprising:
(A) culturing a coryneform bacterium having an L-amino acid producing ability in a medium; and
(B) collecting the L-amino acid from the medium,
wherein the bacterium has been modified to increase the expression of a gene encoding a phosphate transporter, wherein the increased expression of the gene is obtained by increasing the copy number of the gene and/or modifying an expression control sequence of the gene, wherein said phosphate transporter is selected from the group consisting of:
(a) a protein which comprises the amino acid sequence of SEQ ID NO: 6 or SEQ ID NO: 26,
(b) a protein which comprises all of SEQ ID NO: 6 or 26 except for the substitution, deletion, insertion or addition of 1 to 10 amino acid residues, wherein said protein has phosphate transporter activity,
(c) a protein which comprises all of SEQ ID NO: 6 or 26 except for the replacement of the amino acid residue at a position corresponding to position 246 of SEQ ID NO: 6 with a serine residue, and
(d) a protein which comprises all of SEQ ID NO: 6 or 26 except for (i) the replacement of the amino acid residue at a position corresponding to position 246 of SEQ ID NO: 6 with a serine residue, and (ii) the substitution, deletion, insertion or addition of 1 to 10 amino acid residues, wherein said protein has phosphate transporter activity.

2. The method according to claim 1 , wherein the gene encoding the phosphate transporter is a DNA selected from the group consisting of:
(a) a DNA comprising the nucleotide sequence of SEQ ID NO: 5 or 25, and
(b) a DNA which is able to hybridize under stringent conditions with the full-length complement of the polynucleotide of SEQ ID NO: 5 or 25, wherein said stringent conditions comprise washing with 1×SSC, 0.1% SDS at 60° C., and wherein said DNA encodes a protein having phosphate transporter activity.

3. The method according to claim 1 , wherein the bacterium is a Corynebacterium bacterium.

4. The method according to claim 3 , wherein the bacterium is Corynebacterium glutamicum.

5. The method according to claim 1 , wherein the L-amino acid is L-glutamic acid.

6. The method according to claim 5 , wherein the L-glutamic acid is monoammonium L-glutamate or monosodium L-glutamate.

7. A method for producing an L-amino acid comprising:
(A) culturing a coryneform bacterium having an L-amino acid producing ability in a medium; and
(B) collecting the L-amino acid from the medium,
wherein the bacterium has been modified to express a gene encoding a phosphate transporter, wherein said phosphate transporter is selected from the group consisting of:
(a) a protein which comprises all of SEQ ID NO: 6 or 26 except for the replacement of the amino acid residue at a position corresponding to position 246 of SEQ ID NO: 6 with a serine residue, and
(b) a protein which comprises all of SEQ ID NO: 6 or 26 except for (i) the replacement of the amino acid residue at a position corresponding to position 246 of SEQ ID NO: 6 with a serine residue, and (ii) the substitution, deletion, insertion or addition of 1 to 10 amino acid residues, wherein said protein has phosphate transporter activity.

8. The method according to claim 7 , wherein the bacterium is a Corynebacterium bacterium.

9. The method according to claim 8 , wherein the coryneform bacterium is Corynebacterium glutamicum.

10. The method according to claim 7 , wherein the L-amino acid is L-glutamic acid.

11. The method according to claim 10 , wherein the L-glutamic acid is monoammonium L-glutamate or monosodium L-glutamate.

Assignees

• Ajinomoto Kk

Inventors

• Hirano Seiko
• Hayashi Kazuyuki

Classifications

• C12P13/14 Primary

  Glutamic acid; Glutamine · CPC title

• C12P13/04

  Alpha- or beta- amino acids {(other amino acids C12P13/005)} · CPC title

• C07K14/34

  from Corynebacterium (G) · CPC title