Methods and compositions to support transplanted tissue integration and innosculation with adipose stromal cells

Patent metadata

Field	Value
Publication number	US-9506035-B2
Application number	US-50209010-A
Country	US
Kind code	B2
Filing date	Oct 13, 2010
Priority date	Oct 13, 2009
Publication date	Nov 29, 2016
Grant date	Nov 29, 2016

Abstract

The present invention generally relates to methods, compositions and uses thereof for enhancing vascularization of a tissue or cell transplant for transplantation into a subject. In particular, one aspect of the present invention provides methods and compositions comprising the use of a population of stromal vascular fraction (SVF) cells to encapsulate or surround a tissue or cell transplant to enhance vascularization of the tissue or cell transplant. Another aspect of the present invention provides methods and compositions for enhancing vascularization of a tissue or cell transplant by combining a population of SVF cells with a tissue or cell transplant to form a transplant mixed with SVF cells. In some embodiments, the SVF cells can be on the surface or embedded within a three-dimensional matrix. In some embodiments, the SVF cells can be generically engineered to secrete therapeutic proteins or pro-angiogenic factors.

First claim

The invention claimed is:

1. A composition comprising a tissue or cell transplant and a population of cells comprising stromal vascular fraction (SVF) cells, wherein the population of cells comprising SVF cells coats at least a portion of the outer surface of the tissue or cell transplant, wherein the SVF cells present on the surface of, or embedded within a three-dimensional matrix comprises microvessel fragments and wherein the population of cells comprising SVF cells are not cultured by incubation for a period of time prior to contracting the tissue of cell transplant.

2. The composition of claim 1 , wherein the population of SVF cells substantially covers the outer surface of the tissue or cell transplant.

3. The composition of claim 2 , wherein a single cell layer or a multiple cell layer of SVF cells covers at least 70% of the outer surface of the tissue or cell transplant.

4. The composition of claim 1 , wherein the population of cells comprising SVF cells is present on the surface of, or embedded within a three-dimensional matrix, wherein the population of SVF cells contacts the tissue of the cell transplant.

5. The composition of claim 1 , wherein the tissue or cell transplant is genetically engineered.

6. The composition of claim 1 , wherein the tissue or cell transplant is a tissue engineered tissue.

7. The composition of claim 1 wherein inosculation is established post-transplantation.

8. The composition of claim 7 wherein establishing inosculation comprises a functional response to a glucose challenge.

Assignees

• Williams Stuart K
• Hoying James Beatty
• Krishnan Laxminarayanan
• Univ Louisville Res Found Inc

Inventors

• Williams Stuart K
• Hoying James Beatty
• Krishnan Laxminarayanan

Classifications

• A61P7/00

  Drugs for disorders of the blood or the extracellular fluid · CPC title

• A61P9/00

  Drugs for disorders of the cardiovascular system · CPC title

• A61P41/00

  Drugs used in surgical methods, e.g. surgery adjuvants for preventing adhesion or for vitreum substitution · CPC title

• A61K35/35 Primary

  Fat tissue; Adipocytes; Stromal cells; Connective tissues (adipose-derived stem cells A61K35/28; collagen A61K38/39) · CPC title

• A61K35/28

  Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells · CPC title