Methods and materials for increasing potency of cells

Patent metadata

Field	Value
Publication number	US-9506031-B2
Application number	US-201414563459-A
Country	US
Kind code	B2
Filing date	Dec 8, 2014
Priority date	Oct 22, 2004
Publication date	Nov 29, 2016
Grant date	Nov 29, 2016

Abstract

Disclosed herein are methods and materials for producing a more developmentally potent cell from a less developmentally potent cell. Specifically exemplified herein are methods that comprise introducing an expressible dedifferentiating polynucleotide sequence into a less developmentally potent cell, wherein the transfected less developmentally potent cell becomes a more developmentally potent cell capable of differentiating to a less developmentally potent cell of its lineage of origin or a different lineage.

First claim

What is claimed is:

1. A mesenchymal stem cell possessing increased developmental potency, said mesenchymal stem cell produced by introducing into said mesenchymal stem cell at least one polynucleotide comprising coding regions for oct4 and sox2, wherein expression of said coding regions causes an increase of nanog in the mesenchymal stem cell, and wherein said expression results in higher developmental potency of said mesenchymal stem cell.

2. A cell produced by a method comprising introducing into a mesenchymal cell at least one polynucleotide encoding oct4 and at least one polynucleotide encoding sox2.

Assignees

• Sugaya Kiminobu
• Alvarez Angel
• Univ Central Florida Res Found Inc

Inventors

• Sugaya Kiminobu
• Alvarez Angel

Classifications

• A61P25/28

  for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia · CPC title

• C12N2501/605

  Nanog · CPC title

• C12N2506/1346

  from mesenchymal stem cells · CPC title

• C12N2510/00

  Genetically modified cells · CPC title

• C12N5/0663

  Bone marrow mesenchymal stem cells (BM-MSC) · CPC title