Mechanical cell lysis apparatus

Patent metadata

Field	Value
Publication number	US-9506028-B2
Application number	US-51662410-A
Country	US
Kind code	B2
Filing date	Aug 30, 2010
Priority date	Dec 18, 2009
Publication date	Nov 29, 2016
Grant date	Nov 29, 2016

Abstract

The present invention relates to a cell lysis apparatus and a manufacturing method thereof, and more particularly, to a cell lysis apparatus, which mechanically performs cell lysis, and a manufacturing method thereof. The cell lysis apparatus includes: an inlet port through which fluid containing cells is supplied; a fluid channel defining a passage through which the cell containing fluid supplied from the inlet port flows; a nano blade array including a plurality of nano blades having pointed leading ends and arranged such that the pointed leading ends of the nano blades are oriented in a direction of the fluid supplied through the inlet port to disrupt the cells passing therethrough; and an outlet port through which the disrupted cells and the fluid are discharged.

First claim

The invention claimed is:

1. A mechanical cell lysis apparatus comprising:
an inlet port through which fluid is supplied, wherein the fluid contains cells;
an outlet port through which disrupted cells and the fluid are discharged;
a fluid channel defining a passageway between the inlet port and the outlet port through which the fluid supplied from the inlet port flows, wherein the fluid channel has a first end and a second end, wherein the first end is enlarged from one end of the inlet port, the first end forming an acute angle, and the second end is narrowed at a proximal end of the outlet port;
a flow distribution structure within the fluid channel allowing uniform distribution of the fluid within the fluid channel;
a micro-filter array to remove foreign matter from the fluid, wherein the micro-filter array is downstream of the flow distribution structure; and
a nano blade array including a plurality of nano blades having pointed leading ends and arranged such that the pointed leading ends of the nano blades are oriented in a direction of the fluid supplied through the inlet port to disrupt the cells passing therethrough, wherein the nano blade array is downstream of the micro-filter array.

2. The apparatus according to claim 1 , wherein the plurality of nano blades in the nano blade array is disposed parallel to flow of the fluid supplied through the inlet port.

3. The apparatus according to claim 1 , wherein the nano blade array is disposed across the fluid channel connecting the inlet port to the outlet port.

4. The apparatus according to claim 1 , wherein the micro-filter array comprises at least two rows of micro-filters, one row of micro-filters near the inlet port having a greater distance between micro-filters than the other row of micro-filters.

5. The apparatus according to claim 1 , wherein the inlet port, the nano blade array, the fluid channel and the outlet port are formed on a main panel, and a cover panel is attached to an upper side of the main panel.

6. The apparatus according to claim 5 , wherein the inlet port and the outlet port are placed coplanar with the fluid channel connecting the inlet port to the outlet port.

7. The apparatus according to claim 5 , wherein the cover panel is provided with an inlet tube connected to the inlet port and an outlet tube connected to the outlet port.

8. The apparatus according to claim 5 , wherein the inlet port is configured to be attached to a connector connected to an external device.

9. The apparatus according to claim 8 , wherein the external device comprises a syringe.

10. The apparatus according to claim 1 , wherein the inlet port, the fluid channel, the nano blade array, and the outlet port are formed on a main panel through crystalline wet etching.

11. The apparatus according to claim 1 , wherein the inlet port, the fluid channel, the nano blade array, and the outlet port are formed by injection using stampers corresponding thereto.

Assignees

• Yang Sung
• Yun Sung Sik
• Yoon Sang Youl
• Gwangju Inst Science & Tech

Inventors

• Yang Sung
• Yun Sung Sik
• Yoon Sang Youl

Classifications

• C12M47/06 Primary

  Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material (lysis of microorganisms C12N1/06; extracting or separating nucleic acids from biological samples C12N15/1003) · CPC title

• B02C19/0056

  specially adapted for specific materials not otherwise provided for · CPC title

• G01N33/48

  Biological material, e.g. blood, urine (G01N33/02, G01N33/26, G01N33/44, G01N33/46 take precedence); Haemocytometers (counting blood corpuscules distributed over a surface by scanning the surface G06M11/02) · CPC title

• C12Q1/24 Primary

  Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms · CPC title