Interleukin-10 peptides and antibodies thereof for inhibiting adverse effects of protozoan infection

Patent metadata

Field	Value
Publication number	US-9505836-B2
Application number	US-201414150354-A
Country	US
Kind code	B2
Filing date	Jan 8, 2014
Priority date	Jul 13, 2012
Publication date	Nov 29, 2016
Grant date	Nov 29, 2016

Abstract

The present disclosure is directed to interleukin-10 (IL-10) peptides and isolated antibodies that specifically bind to the IL-10 peptides. The IL-10 peptides and the isolated antibodies may be administered alone or as an animal feed additive to treat gastrointestinal protozoan infection in animals.

First claim

What is claimed is:

1. An animal feed comprising from about 0.15 mg/kg to about 50 mg/kg of an animal feed additive comprising an isolated antibody that specifically binds to an interleukin-10 peptide, the interleukin-10 peptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, and SEQ ID NO: 20.

2. The animal feed of claim 1 , wherein the interleukin-10 peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 4, SEQ ID NO: 7, and SEQ ID NO: 10.

3. The animal feed of claim 1 , wherein the isolated antibody is an avian egg antibody.

4. The animal feed of claim 3 , wherein the avian egg antibody is concentrated in the egg yolk.

5. The animal feed of claim 4 , wherein the egg yolk is dried.

6. The animal feed of claim 1 , wherein the antibody is a monoclonal antibody, a synthetic antibody, or a genetically engineered antibody.

7. An animal feed additive comprising egg yolk, the egg yolk comprising anti-interleukin-10 antibodies, wherein the anti-interleukin-10 antibodies specifically bind to an interleukin-10 peptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, and SEQ ID NO: 20.

8. The animal feed additive of claim 7 , wherein the anti-interleukin-10 antibodies in the egg yolk comprise total 1 to 10% by weight of the total IgY in the egg yolk.

9. The animal feed additive of claim 7 , wherein the interleukin-10 peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 4, SEQ ID NO: 7, and SEQ ID NO: 10.

10. An animal feed comprising animal feed additive comprising egg yolk, the egg yolk comprising anti-IL-10 antibodies, wherein the anti-IL-10 antibodies specifically bind to an interleukin-10 peptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, and SEQ ID NO: 20.

11. The animal feed of claim 10 , wherein the anti-interleukin-10 antibodies in the egg yolk comprise total 1 to 10% by weight of the total IgY in the egg yolk.

12. The animal feed of claim 10 , wherein the interleukin-10 peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 4, SEQ ID NO: 7, and SEQ ID NO: 10.

Assignees

• Wisconsin Alumni Res Found

Inventors

• Sand Jordan Marshall
• Cook Mark Eric

Classifications

• A61K38/2066

  IL-10 · CPC title

• C07K2317/34

  Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues · CPC title

• C07K2317/23

  from birds · CPC title

• A61P33/02

  Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis · CPC title

• C07K14/5428

  IL-10 · CPC title