Plant CO2 sensors, nucleic acids encoding them, and methods for making and using them

Patent metadata

Field	Value
Publication number	US-9505811-B2
Application number	US-201414198551-A
Country	US
Kind code	B2
Filing date	Mar 5, 2014
Priority date	Apr 27, 2007
Publication date	Nov 29, 2016
Grant date	Nov 29, 2016

Abstract

The invention provides compositions and methods for manipulating the exchange of water and/or carbon dioxide (CO 2 ) through plant stomata by controlling CO 2 sensor genes. The invention provides compositions and methods for enhancing or optimizing biomass accumulation in a plant. The invention provides compositions and methods for opening or closing a stomatal pore on a guard cell in the epidermis of a plant. The invention provides compositions and methods for increasing or decreasing oxygenation efficiency and/or carbon fixation in a guard cell in the epidermis of a plant by manipulating expression of a ribulose-1,5-bisphosphate carboxylase/oxygenase. The invention provides promoters for regulating expression of a nucleic acid in a plant guard cell.

First claim

What is claimed is:

1. An isolated, synthetic or recombinant nucleic acid or polynucleotide, operably linked to a heterologous nucleic acid sequence, wherein the nucleic acid or polynucleotide:
(a) has a nucleic acid sequence having at least about 95% sequence identity to SEQ ID NO: 10 and/or SEQ ID NO: 11, and has guard cell-specific promoter activity; comprises a transcriptional regulatory region having guard cell-specific activity; comprises a guard cell-specific promoter; or, comprises guard cell-specific transcriptional regulatory region; or
(b) wherein the nucleic acid or polynucleotide comprises:
(i) the nucleotide sequence of SEQ ID NO:10 from nucleotide at position 833 to nucleotide at position 1716, or
(ii) the nucleotide sequence of SEQ ID NO:10 from nucleotide at position 1251 to nucleotide at position 1716.

2. An expression cassette, plasmid, recombinant virus, vector, cosmid or artificial chromosome comprising or having contained therein the isolated, synthetic or recombinant nucleic acid or polynucleotide operably linked to the heterologous nucleic acid sequence of claim 1 .

3. A transduced or transformed cell comprising or having contained therein a heterologous nucleic acid or polynucleotide comprising or consisting of the isolated, synthetic or recombinant nucleic acid or polynucleotide operably linked to the heterologous nucleic acid sequence of claim 1 .

4. A transduced or transformed cell comprising or having contained therein a heterologous nucleic acid or polynucleotide comprising or consisting of the expression cassette, plasmid, recombinant virus, vector, cosmid or artificial chromosome of claim 2 .

5. A plant cell, a plant organ, a plant leaf, a plant fruit or a seed comprising or having contained therein a heterologous nucleic acid or polynucleotide comprising or consisting of the isolated, synthetic or recombinant nucleic acid or polynucleotide operably linked to the heterologous nucleic acid sequence of claim 1 .

6. A plant cell, a plant organ, a plant leaf, a plant fruit or a seed comprising or having contained therein a heterologous nucleic acid or polynucleotide comprising or consisting of the expression cassette, plasmid, recombinant virus, vector, cosmid or artificial chromosome of claim 2 .

7. The plant cell, plant organ, plant leaf, plant fruit or seed of claim 5 , wherein the plant cell, plant organ, plant leaf, plant fruit or seed is or is derived from: (a) a dicotyledonous or monocotyledonous plant; (b) a wheat, oat, rye, barley, rice, sorghum , maize (corn), tobacco, a legume, a lupins, potato, sugar beet, pea, bean, soybean (soy), a cruciferous plant, a cauliflower, rape (or rapa or canola), cane (sugarcane), flax, cotton, palm, sugar beet, peanut, a tree, a poplar, a lupin, a silk cotton tree, desert willow, creosote bush, winterfat, balsa, ramie, kenaf, hemp, roselle, jute, or sisal abaca; or (c) a species from the genera Anacardium, Arachis, Asparagus, Atropa, Avena, Brassica, Citrus, Citrullus, Capsicum, Carthamus, Cocos, Coffea, Cucumis, Cucurbita, Daucus, Elaeis, Fragaria, Glycine, Gossypium, Helianthus, Heterocallis, Hordeum, Hyoscyamus, Lactuca, Linum, Lolium, Lupinus, Lycopersicon, Malus, Manihot, Majorana, Medicago, Nicotiana, Olea, Oryza, Panieum, Pannisetum, Persea, Phaseolus, Pistachia, Pisum, Pyrus, Prunus, Raphanus, Ricinus, Secale, Senecio, Sinapis, Solanum, Sorghum, Theobromus, Trigonella, Triticum, Vicia, Vitis, Vigna or Zea.

8. The plant cell, plant organ, plant leaf, plant fruit or seed of claim 6 , wherein the plant cell, plant organ, plant leaf, plant fruit or seed is or is derived from: (a) a dicotyledonous or monocotyledonous plant; (b) a wheat, oat, rye, barley, rice, sorghum , maize (corn), tobacco, a legume, a lupins, potato, sugar beet, pea, bean, soybean (soy), a cruciferous plant, a cauliflower, rape (or rapa or canola), cane (sugarcane), flax, cotton, palm, sugar beet, peanut, a tree, a poplar, a lupin, a silk cotton tree, desert willow, creosote bush, winterfat, balsa, ramie, kenaf, hemp, roselle, jute, or sisal abaca; or (c) a species from the genera Anacardium, Arachis, Asparagus, Atropa, Avena, Brassica, Citrus, Citrullus, Capsicum, Carthamus, Cocos, Coffea, Cucumis, Cucurbita, Daucus, Elaeis, Fragaria, Glycine, Gossypium, Helianthus, Heterocallis, Hordeum, Hyoscyamus, Lactuca, Linum, Lolium, Lupinus, Lycopersicon, Malus, Manihot, Majorana, Medicago, Nicotiana, Olea, Oryza, Panieum, Pannisetum, Persea, Phaseolus, Pistachia, Pisum, Pyrus, Prunus, Raphanus, Ricinus, Secale, Senecio, Sinapis, Solanum, Sorghum, Theobromus, Trigonella, Triticum, Vicia, Vitis, Vigna or Zea.

9. The isolated, synthetic or recombinant nucleic acid or polynucleotide operably linked to the heterologous nucleic acid sequence of claim 1 , wherein the heterologous sequence comprises: (a) a nucleic acid encoding a CO 2 Sen protein; (b) a CO 2 Sen gene or transcript; or (c) a nucleic acid encoding a carbonic anhydrase polypeptide.

10. The isolated, synthetic or recombinant nucleic acid or polynucleotide operably linked to the heterologous nucleic acid sequence of claim 1 , wherein the heterologous nucleic acid sequence comprises:
(a) a nucleic acid encoding a CO 2 Sen (CO 2 sensor) protein;
(b) a CO 2 Sen gene, transcript or message; or
(c) a nucleic acid encoding a carbonic anhydrase (CA) polypeptide; wherein optionally said CO 2 Sen gene or transcript comprises:
a nucleic acid or polynucleotide having at least about 95%, 96%, 97%, 98% or 99% sequence identity to SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34 and/or SEQ ID NO:35.

11. A method for selectively expressing a polypeptide, protein, gene or nucleic acid in a plant guard cell, comprising: inserting, transforming or placing into the plant guard cell a nucleic acid expressing the polypeptide, protein, gene or nucleic acid, wherein the nucleic acid expressing the polypeptide, protein, gene or nucleic acid comprises: (i) the polynucleotide or nucleic acid of claim 1 .

12. A method for selectively expressing a polypeptide, protein, gene or nucleic acid in a plant, plant organ, plant leaf, plant fruit or seed, comprising: inserting, transforming or placing into a cell of the plant, plant organ, plant leaf, plant fruit or seed, a nucleic acid expressing the polypeptide, protein, gene or nucleic acid, wherein the nucleic acid expressing the polypeptide, protein, gene or nucleic acid comprises: (i) the polynucleotide or nucleic acid of claim 1 .

13. The isolated, synthetic or recombinant nucleic acid or polynucleotide operably linked to the heterologous nucleic acid sequence of claim 1 , wherein the nucleic acid or polynucleotide has a nucleic acid sequence having at least 98% sequence identity to SEQ ID NO: 10 and/or SEQ ID NO: 11.

14. The isolated, synthetic or recombinant nucleic acid or polynucleotide operably linked to the heterologous nucleic acid sequence of claim 13 , wherein the nucleic acid or polynucleotide has a nucleic acid sequence as set forth in SEQ ID NO: 10.

15. The isolated, synthetic or recombinant nucleic acid or polynucleotide operably linked to the heterologous sequence of claim 13 , wherein the nucleic acid or polynucleotide has a nucleic acid sequence as set forth in SEQ ID NO: 11.

16. The method of claim 12 , wherein the nucleic acid or polynucleotide has a nucleic acid sequence having at least 98% sequence identity to SEQ ID NO: 10 a

Assignees

• Univ California

Inventors

• Schroeder Julian I
• Israelsson-Nordstrom Maria
• Kuhn Josef M
• Yang Yingzhen
• Hu Honghong
• Boisson-Dernier Aurelien

Classifications

• C12N15/8273 Primary

  for drought, cold, salt resistance · CPC title

• C12N15/8218

  Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS] · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C12N15/8225

  Leaf-specific, e.g. including petioles, stomata · CPC title

• C12N15/8261

  with agronomic (input) traits, e.g. crop yield · CPC title