Library preparation methods and compositions and uses therefor
US-12065643-B2 · Aug 20, 2024 · US
US9499863B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9499863-B2 |
| Application number | US-201314028319-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 16, 2013 |
| Priority date | Dec 5, 2007 |
| Publication date | Nov 22, 2016 |
| Grant date | Nov 22, 2016 |
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The present invention is directed to methods and compositions for long fragment read sequencing. The present invention encompasses methods and compositions for preparing long fragments of genomic DNA, for processing genomic DNA for long fragment read sequencing methods, as well as software and algorithms for processing and analyzing sequence data.
Opening claim text (preview).
The invention claimed is: 1. A method of analyzing genomic DNA in a sample, the method comprising: (a) dividing the sample into a plurality of separate aliquots; (b) amplifying fragments of the genomic DNA in the separate aliquots to produce a plurality of amplicons, wherein the amplifying is conducted with a population of dNTPs containing dNTP analogs, whereby a number of nucleotides in the amplicons are nucleotide analogs; (c) removing nucleotide analogs incorporated into th…
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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