Heteroarylcyanine dyes with sulfonic acid substituents
US-9315864-B2 · Apr 19, 2016 · US
US9499862B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9499862-B2 |
| Application number | US-201514667869-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 25, 2015 |
| Priority date | Aug 25, 2010 |
| Publication date | Nov 22, 2016 |
| Grant date | Nov 22, 2016 |
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In various embodiments, the present invention provides fluorescent dyes that are linked to another species through an amino acid or peptide linker. In an exemplary embodiment, the dye is linked to a polyphosphate nucleic acid through an amino acid or peptide linker. These conjugates find use in single molecule DNA sequencing and other applications. In various embodiments, the dye moiety is a cyanine dye. Cyanine dyes that are highly charged, such as those including multiple sulfonate, alkylsulfonate, carboxylate and/or alkylcarboxylate moieties are examples of cyanine dyes of use in the compounds of the invention.
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What is claimed is: 1. A fluorescent dye having the formula: wherein R l is a fluorescent dye moiety; (AA) n is an amino acid linker; n is a selected from the integers 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 and 12, and when n is two or greater, each n amino acid is independently selected; X is a polyvalent moiety, comprising a moiety having…
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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